MAM-6E7, MAM-3E7, MAM-6G7 (Antirecombinant Human Trypsin-2)

摘要

Antigen Used for Immunization: The recombinant human Trypsin-2 (rh-Trypsin 2) expressed in Escherichia coli used for immunization was purchased from RayBiotech (Norcross, GA). Method of Immunization: Six to eight-week-old four to five female BALB/c mice were immunized with 50 μg of rh-Trypsin 2 in phosphate-buffered saline (PBS; 10mM K_2HPO_4, 0.15M NaCl, pH 7.2) intra-peritoneally. Antigen was emulsified with an equal volume of adjuvants. The first injection was administered with complete Freund's adjuvant. The other injections were administered with incomplete Freund's adjuvant. Immunizations were done every 2 weeks until the desired immune response was achieved. Mice were bled through the tail vein, and sera were tested for rh-Trypsin 2-specific antibody activity by indirect enzyme-linked immunosorbent assay (ELISA). Mice with the highest antibody titers were selected for fusion and received a final intravenous booster injection of rh-Trypsin 2 without adjuvant in PBS 4 days before fusion.