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Environmental DNADNA enables detection of terrestrial mammals from forest pond water

机译：环境 DNA DNA能够检测来自森林池塘水的地面哺乳动物

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Abstract > Terrestrial animals must have frequent contact with water to survive, implying that environmental <fc>DNA</fc> ( <fc>eDNA</fc> ) originating from those animals should be detectable from places containing water in terrestrial ecosystems. Aiming to detect the presence of terrestrial mammals using forest water samples, we applied a set of universal <fc>PCR</fc> primers (MiMammal, a modified version of fish universal primers) for metabarcoding mammalian <fc>eDNA</fc> . The versatility of MiMammal primers was tested in silico and by amplifying <fc>DNA</fc> s extracted from tissues. The results suggested that MiMammal primers are capable of amplifying and distinguishing a diverse group of mammalian species. In addition, analyses of water samples from zoo cages of mammals with known species composition suggested that MiMammal primers could successfully detect mammalian species from water samples in the field. Then, we performed an experiment to detect mammals from natural ecosystems by collecting five 500‐ml water samples from ponds in two cool‐temperate forests in Hokkaido, northern Japan. MiMammal amplicon libraries were constructed using <fc>eDNA</fc> extracted from water samples, and sequences generated by Illumina MiSeq were subjected to data processing and taxonomic assignment. We thereby detected multiple species of mammals common to the sampling areas, including deer ( Cervus nippon ), mouse ( Mus musculus ), vole ( Myodes rufocanus ), raccoon ( Procyon lotor ), rat ( Rattus norvegicus ) and shrew ( Sorex unguicula </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：id =“men12690-abs-0001”> <标题类型=“main”>抽象</ title> < P>陆地动物必须与水频繁接触以存活，暗示来自那些动物的环境<FC> DNA </ FC>（<FC> EDNA </ FC>）应该可检测含有陆地生态系统中的水的地方。旨在检测使用森林水样的陆地哺乳动物的存在，我们应用了一组Universal <Fc> PCR </ Fc>引物（MIMammal，Fish Universion Primers的Fish Universal Primers），用于哺乳动物<Fc> EDNA </ FC> 。在硅引物中的含咪酰位基底漆的多功能性在硅中进行测试，并通过从组织中提取的<Fc> DNA </ Fc> S。结果表明，思维引物能够放大和区分各种哺乳动物种类。此外，从已知物种组成的哺乳动物动物笼中的水样的分析表明，莫因子引物可以成功地从场中的水样中检测哺乳动物。然后，我们通过在日本北部北海道的两个凉爽的温带森林中收集来自池塘的五种500ml水样来检测来自天然生态系统的哺乳动物的实验。使用从水样中提取的<FC> EDNA </ FC>构建摩尼汞扩增子文库，并通过Illumina Miseq产生的序列进行数据处理和分类作业。因此，我们检测到对采样区域的多种哺乳动物，包括鹿（宫颈Nippon </ i>），鼠标（亩肌肉</ i>），vole（ myodes rufocanus </ i >），浣熊（ procyon lotor </ i>），大鼠（ rattus norvegicus </ i>）和shrew（ sorex unguicula </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-25257/'>《Molecular ecology resources》</a> <b style="margin: 0 2px;">|</b><span>2017年第6期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ushio Masayuki&option=202" target="_blank" rel="nofollow">Ushio Masayuki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fukuda Hisato&option=202" target="_blank" rel="nofollow">Fukuda Hisato;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Inoue Toshiki&option=202" target="_blank" rel="nofollow">Inoue Toshiki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Makoto Kobayashi&option=202" target="_blank" rel="nofollow">Makoto Kobayashi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kishida Osamu&option=202" target="_blank" rel="nofollow">Kishida Osamu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sato Keiichi&option=202" target="_blank" rel="nofollow">Sato Keiichi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Murata Koichi&option=202" target="_blank" rel="nofollow">Murata Koichi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nikaido Masato&option=202" target="_blank" rel="nofollow">Nikaido Masato;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sado Tetsuya&option=202" target="_blank" rel="nofollow">Sado Tetsuya;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sato Yukuto&option=202" target="_blank" rel="nofollow">Sato Yukuto;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Takeshita Masamichi&option=202" target="_blank" rel="nofollow">Takeshita Masamichi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Iwasaki Wataru&option=202" target="_blank" rel="nofollow">Iwasaki Wataru;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamanaka Hiroki&option=202" target="_blank" rel="nofollow">Yamanaka Hiroki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kondoh Michio&option=202" target="_blank" rel="nofollow">Kondoh Michio;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Miya Masaki&option=202" target="_blank" rel="nofollow">Miya Masaki;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Center for Ecological ResearchKyoto UniversityOtsu Japan;</p> <p>Department of Environmental Solution TechnologyRyukoku UniversityOtsu Japan;</p> <p>Department of Environmental Solution TechnologyRyukoku UniversityOtsu Japan;</p> <p>Teshio Experimental ForestHokkaido UniversityHokkaido Japan;</p> <p>Teshio Experimental ForestHokkaido UniversityHokkaido Japan;</p> <p>Okinawa Churashima Research CenterOkinawa Japan;</p> <p>College of Bioresource SciencesNihon UniversityKanagawa Japan;</p> <p>School of Life Science and TechnologyTokyo Institute of TechnologyTokyo Japan;</p> <p>Natural History Museum and InstituteChiba Japan;</p> <p>Tohoku Medical Megabank OrganizationTohoku UniversityMiyagi Japan;</p> <p>Department of Biological SciencesThe University of TokyoTokyo Japan;</p> <p>Department of Biological SciencesThe University of TokyoTokyo Japan;</p> <p>Department of Environmental Solution TechnologyRyukoku UniversityOtsu Japan;</p> <p>Department of Environmental Solution TechnologyRyukoku UniversityOtsu Japan;</p> <p>Natural History Museum and InstituteChiba Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=environmental DNA&option=203" rel="nofollow">environmental DNA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Forest&option=203" rel="nofollow">Forest;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Illumina MiSeq&option=203" rel="nofollow">Illumina MiSeq;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mammal&option=203" rel="nofollow">Mammal;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabarcoding&option=203" rel="nofollow">metabarcoding;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=terrestrial ecosystem&option=203" rel="nofollow">terrestrial ecosystem;</a> </p> <div class="translation"> 机译：环境DNA;森林;illumina miseq;哺乳动物;成立;陆地生态系统; </div> </li> </ul> </div> </div> <div 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href="/journal-cn-6571/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国医科大学学报 </a> </span> <span> . 2017</span><span>,第010期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-central-south-university-medical-science_thesis/0201235053996.html">利用补丁甲基化技术构建特殊报告载体检测DNA甲基化对FCER1G基因启动子的调控活性</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=梁云生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 梁云生</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵明&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,赵明</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=梁功平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,梁功平</a> <span> <a href="/journal-cn-11891/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中南大学学报（医学版） </a> </span> <span> . 2013</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_shanghai-medical-laboratory_thesis/0201267177247.html">FCM检测胸腹水脱落细胞DNA含量的探讨</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=方国安&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 方国安</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=金秀国&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,金秀国</a> <span> <a href="/journal-cn-50922/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 上海医学检验杂志 </a> </span> <span> . 1999</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-shanghai-second-medical-university_thesis/0201265772789.html">胃癌细胞核仁组成区和DNA含量FCM检测及相互关系的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡丹毅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 胡丹毅</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=庄亚华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,庄亚华</a> <span> <a href="/journal-cn-50545/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 上海第二医科大学学报 </a> </span> <span> . 1991</span><span>,第002期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104605729.html">表达TNF受体:IgG1 Fc嵌合蛋白的DNA型Semliki森林脑炎病毒载体</a> <b>[P]</b> . <span> 中国专利： CN1408875A </span> <span> . 2003-04-09</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130427693709.html">method for the detection of dna einzelstrangbru00fcchen</a> <b>[P]</b> . <span> 外国专利：  AT477335T </span> <span> . 2010-08-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: 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