A preliminary attempt to establish multiple stress response profiles of human skin fibroblasts exposed to mild or severe stress during ageing in vitro

摘要

Optimal stress response (SR) is an essential aspect of the property of dynamic homeostasis of all biologica systems, including cells in culture. Whereas severe stress can induce the so-called stress-induced premature senescence (SIPS), a model developed by Olivier Toussaint, mild stress can strengthen homeodynamics and car postpone senescence through the phenomenon of hormesis. We have attempted to establish multiple stres: response profiles (SRP) of early passage young and late passage senescent human facial skin fibroblasts, FSF-1 exposed to either mild (41 degrees C) and severe (43 degrees C) heat shock for 1 h, or to mild (2%) and severe (0%) serurt deprivation for up to 48 h. The results obtained show that FSF-1 cells exposed to two different intensities of stres: from two different stressors separately have differential SRP to mild and severe stress, which also vary sig nificantly between young and senescent cells. Establishing multiple and differential SRP to mild and severe stres: may facilitate distinguishing between the mild stress-induced beneficial hormetic effects and the harmful effect of severe stress.