Protocol identification and preservation of myxozoan parasites for microscopy with silver nitrate (Klein's dry) staining technique

摘要

The present study, discusses a first report of staining techniques with silver nitrate (AgNO3) stain for the preservation and identification of myxozoans globally. The silver nitrate stain was used to prepare permanent slide preparation of myxozoans with some adaptations made in our laboratory. Fresh air dried smear were stained with silver nitrate stain ensuing dark brown color polar capsules and light brown color of spore-wall. The stain is everlasting for years differing to other stains like Geimsa, iron-heamotoxylin Zeihl-Neelsen Blue, and trichome stains.