• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microscopy research and technique >Visualization of the native shape of bodipy‐labeled DNA in Escherichia coliEscherichia coli by correlative microscopy
【24h】

Visualization of the native shape of bodipy‐labeled DNA in Escherichia coliEscherichia coli by correlative microscopy

机译：通过相关显微镜可视化大肠杆菌（Escherichia coli）中Bypipy标记的DNA的本地形状的可视化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract > The native shape and intracellular distribution of newly synthesized DNA was visualized by correlative (light and electron) microscopy in ice embedded whole cells of Escherichia coli . For that purpose, the commercially available modified nucleoside triphosphate named BODIPY? FL‐14‐dUTP was enzymatically incorporated in vivo into the genome of E. coli mutant K12 strain, which cannot synthesize thymine. The successful incorporation of this thymidine analogue was confirmed first by fluorescence microscope, where the cells were stained in the typical for bodipy green color. Later the preselected labeled E. coli were observed by Hilbert Differential Transmission Electron Microscope (HDC TEM) and the distribution of elemental boron (contained in bodipy) was visualized at high‐resolution by an electron spectroscopic imaging (ESI) technique. The practical detection limit of boron was found to be around 5 ～ 10 mmol/kg in area of 0.1 μm 2 , which demonstrated that ESI is a suitable approach to study the cytochemistry and location of labeled nucleic fragments within the cytoplasmic chromosomal area. In addition, the fine cellular fibrous and chromosomal ultrastructures were revealed in situ by combing of phase‐plate HDC TEM and ESI. The obtained results conclude that the correlation between fluorescent microscopy with phase‐plate HDC TEM and ESI is a powerful approach to explore the structural and conformation dynamics of DNA replication machinery in frozen cells close to the living state. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”> <标题类型=“main”>抽象</ title> >新合成的本地形状和细胞内分布通过冰嵌入式整体细胞的相关（光和电子）显微镜在大肠杆菌的整个细胞中进行可视化DNA。为此目的，商业上可获得的修饰的核苷三磷酸盐命名为BODIPY？将FL-14-DUTP酶促掺入中的进入 e的基因组中。 Coli </ i>突变体K12菌株，其不能合成胸腺嘧啶。首先通过荧光显微镜确认该胸苷类似物的成功掺入，其中细胞染色在典型的Bibipy绿色。后来预选标记为 e。通过Hilbert差分透射电子显微镜（HDC TEM）观察到COLI </ i>，通过电子光谱成像（ESI）技术在高分辨率以高分辨率可视化元素硼（包含在BODIPY中）的分布。发现硼的实际检测极限为约5〜10mmol / kg，面积为0.1μm 2 </ sup>，表明ESI是研究细胞化学和标记核片段内的细胞化学和位置的合适方法细胞质染色体区域。另外，通过梳理相板HDC TEM和ESI，揭示了细胞纤维和染色体超微结构的原位纤维状和染色体超微结构。所得结果得出结论，荧光显微镜与相板HDC TEM和ESI之间的相关性是探讨靠近生活状态的冷冻细胞中DNA复制机械的结构和构象动态的强大方法。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-24994/'>《Microscopy research and technique》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Loukanov Alexandre&option=202" target="_blank" rel="nofollow">Loukanov Alexandre;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mladenova Polina&option=202" target="_blank" rel="nofollow">Mladenova Polina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Toshev Svetlin&option=202" target="_blank" rel="nofollow">Toshev Svetlin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Udono Hibiki&option=202" target="_blank" rel="nofollow">Udono Hibiki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nakabayashi Seiichiro&option=202" target="_blank" rel="nofollow">Nakabayashi Seiichiro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Verkade Paul&option=202" target="_blank" rel="nofollow">Verkade Paul;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Graduate School of Science and EngineeringSaitama UniversityShimo–Ohkubo 255 Sakura–Ku Saitama 338‐8570 Japan;</p> <p>Laboratory of Eng. NanoBiotechnology Department of Engineering GeoecologyUniversity of Mining and Geology “St. Ivan Rilski”Sofia 1700 Bulgaria;</p> <p>Laboratory of Eng. NanoBiotechnology Department of Engineering GeoecologyUniversity of Mining and Geology “St. Ivan Rilski”Sofia 1700 Bulgaria;</p> <p>Graduate School of Science and EngineeringSaitama UniversityShimo–Ohkubo 255 Sakura–Ku Saitama 338‐8570 Japan;</p> <p>Graduate School of Science and EngineeringSaitama UniversityShimo–Ohkubo 255 Sakura–Ku Saitama 338‐8570 Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/19294.html" title="显微镜">显微镜;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bodipy‐labeled DNA&option=203" rel="nofollow">bodipy‐labeled DNA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=correlative microscopy&option=203" rel="nofollow">correlative microscopy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HDC TEM&option=203" rel="nofollow">HDC TEM;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ice embedded E. coli&option=203" rel="nofollow">ice embedded E. coli;</a> </p> <div class="translation"> 机译：Bodipy标记的DNA;相关显微镜;HDC TEM;冰嵌入式大肠杆菌; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023680155.html">Visualization of the native shape of bodipy‐labeled DNA in <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coliEscherichia coli by correlative microscopy</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Loukanov Alexandre&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Loukanov Alexandre,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mladenova Polina&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Mladenova Polina,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Toshev Svetlin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Toshev Svetlin,</a> <a href="/journal-foreign-24994/" target="_blank" rel="nofollow" class="tuijian_authcolor">Microscopy research and technique .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过相关显微镜可视化<i XMLNS =“http://www.wiley.com/namespace/wiley”>大肠杆菌（Escherichia coli）中Bypipy标记的DNA的本地形状的可视化</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881205.html">Enhancement of? <sc xmlns="http://www.wiley.com/namespaces/wiley">l</sc>l ‐phenylalanine production in <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coliEscherichia coli by heterologous expression of <i xmlns="http://www.wiley.com/namespaces/wiley">VitreoscillaVitreoscilla hemoglobin</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Wei‐Bin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wu Wei‐Bin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Xiao‐Lei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guo Xiao‐Lei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Ming‐Liang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Ming‐Liang,</a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：增强？ <sc xmlns =“http://www.wiley.com/namespaces/wiley”> l </ sc> l -phenylanine生产在<i xmlns =“http://www.wiley.com/namespaces/wiley”> Escherichia Coli </ i>通过<i XMLNS =“http://www.wiley.com/namespaces/wiley”> Vitreoscilla </ i> Vitreoscilla血红蛋白的异源表达</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229266.html">Within‐host evolution <i xmlns="http://www.wiley.com/namespaces/wiley">versusversus immigration as a determinant of <i xmlns="http://www.wiley.com/namespaces/wiley">Escherichia coliEscherichia coli diversity in the human gastrointestinal tract</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dixit Ojas V. A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Dixit Ojas V. A.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=OBrien Claire L.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">OBrien Claire L.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pavli Paul&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pavli Paul,</a> <a href="/journal-foreign-22868/" target="_blank" rel="nofollow" class="tuijian_authcolor">Environmental microbiology .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在主机进化中<i xmlns =“http://www.wiley.com/namespaces/wiley”>与移民为<i xmlns =“http://www.wiley.com/的决定因素 名称空间/ Wiley“>大肠杆菌Coli </ i>大肠杆菌在人胃肠道中的多样性</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040005061305.html">Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rory M. Watt&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rory M. Watt,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jing Wang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jing Wang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meikid Leong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Meikid Leong,</a> <span>2007</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：可视化大肠杆菌的蛋白质组：一种高效且通用的方法用荧光蛋白基因标记染色体编码DNA序列（CDS）</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100037499829.html ">Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Watt, Rory M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Watt, Rory M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang, Jing&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang, Jing,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Leong, Meikid&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Leong, Meikid,</a> <span>2007</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：可视化大肠杆菌的蛋白质组：一种高效且通用的方法，用荧光蛋白基因标记染色体编码DNA序列（CDS）</span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704023680155','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche"> <!-- <a href="javascript:void(0);" onclick="link_analysis('/shoppingcart/auth/list.html',this)">购物车</a>--> </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704023680155down" data-source="7," data-out-id="c0HVjanfPVi8IlI5pkTkkg==," data-f-source-id="7" data-title="Visualization of the native shape of bodipy‐labeled DNA in <i xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia coliEscherichia coli by correlative microscopy" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="24994" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Visualization of the native shape of bodipy‐labeled DNA in <i xmlns='http://www.wiley.com/namespaces/wiley'>Escherichia coliEscherichia coli by correlative microscopy"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>