Cloning, purification and enzymatic assay of streptokinase gene from Streptococcus pyogenes in Escherichia coli

Raee Mohammad J.; Ghasemian Abdollah; Maghami Samira; Ghoshoon Mohammad B.; Ghasemi Younes

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Cloning, purification and enzymatic assay of streptokinase gene from Streptococcus pyogenes in Escherichia coli

机译：从大肠杆菌中链球菌化合物中链球菌酶基因的克隆，纯化和酶法测定

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BACKGROUND: Streptokinase is an important therapeutic enzyme that acts as a fibrinolytic agent and use in the treatment of multi serious and life threatening diseases such as pulmonary embolism and acute myocardial infarction.

机译：背景：链激酶是一种重要的治疗酶，其充当纤维蛋白溶解剂，并用于治疗多重和危及肺栓塞等肺栓塞和急性心肌梗死的治疗。

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来源
《Minerva biotecnologica》 |2017年第1期|共6页
作者
Raee Mohammad J.; Ghasemian Abdollah; Maghami Samira; Ghoshoon Mohammad B.; Ghasemi Younes;
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作者单位

Shiraz Univ Med Sci Pharmaceut Sci Res Ctr Shiraz Iran;

Urmia Univ Med Sci Sch Pharm Dept Pharmaceut Biotechnol Orumiyeh Iran;

Shiraz Univ Med Sci Pharmaceut Sci Res Ctr Shiraz Iran;

Shiraz Univ Med Sci Pharmaceut Sci Res Ctr Shiraz Iran;

Shiraz Univ Med Sci Pharmaceut Sci Res Ctr Shiraz Iran;

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原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;分子生物学;
关键词
escherichia coli; Fibrinolytic agents; Molecular cloning; Streptococcus pyogenes; Streptokinase;

机译：大肠杆菌;纤维蛋白溶解剂;分子克隆;链球菌的细胞;链孢菌素;

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专利

1. Cloning, purification and enzymatic assay of streptokinase gene from Streptococcus pyogenes in Escherichia coli [J] . Raee Mohammad J., Ghasemian Abdollah, Maghami Samira, Minerva biotecnologica . 2017,第1期

机译：从大肠杆菌中链球菌化合物中链球菌酶基因的克隆，纯化和酶法测定
2. Cloning and expression in Escherichia coli of the streptolysin O determinant from Streptococcus pyogenes: characterization of the cloned streptolysin O determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins. [J] . M Kehoe, K N Timmis Infection and immunity . 1984,第3期

机译：化脓性链球菌的链球菌溶血素O决定簇的克隆和在大肠杆菌中的表达：克隆的链球菌溶血素O决定簇的表征，并证明与其他硫醇活化毒素的决定簇不存在实质同源性。
3. Purification of recombinant hyaluronan lyase of Streptococcus pyogenes bacteriophage H4489A expressed in Escherichia coli and its application for the specific determination of hyaluronan concentration [J] . Pei-Fen Yang, Cheng-Kang Lee Carbohydrate Polymers: Scientific and Technological Aspects of Industrially Important Polysaccharides . 2006,第2期

机译：大肠杆菌表达的化脓性链球菌噬菌体H4489A重组透明质酸裂合酶的纯化及其在透明质酸浓度的特异性测定中的应用
4. Integrating proteomics, genomics, and bioinformatics tools to define unique features of the clonal M1T1 strain of Streptococcus pyogenes [C] . Ramy K. Aziz, Malak Kot Lancefield International Symposium on Streptococci and Streptococcal Diseases . 2006

机译：整合蛋白质组学，基因组学和生物信息学工具，以定义克隆性M1T1菌株的独特特征
5. Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26: Affinity protein purification, molecular cloning, sequence of the genes, and expression in Escherichia coli. [D] . Ma, Grace Tak-Yi. 1993

机译：嗜酸乳杆菌R-26的异二聚体脱氧鸟苷/脱氧腺苷激酶：亲和蛋白纯化，分子克隆，基因序列和在大肠杆菌中的表达。
6. Highly Effective Renaturation of a Streptokinase from Streptococcus pyogenes DT7 as Inclusion Bodies Overexpressed in Escherichia coli [O] . Sy Le Thanh Nguyen, Dinh Thi Quyen, Hong Diep Vu -1

机译：化脓性链球菌DT7的链激酶作为在大肠杆菌中过表达的包涵体的高效复性
7. High level expression of Streptococcus pyogenes erythrogenic toxin A (SPE A) in Escherichia coli and its rapid purification by HPLC [O] . M Yamamoto 1995

机译：高层细胞的细胞霉菌性毒素（SPE A）的高水平表达及其通过HPLC的快速纯化

Cloning, purification and enzymatic assay of streptokinase gene from Streptococcus pyogenes in Escherichia coli

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