Development and validation of high performance liquid chromatographic method for determination of gentisic acid and related Renal Cell Carcinoma biomarkers in urine

摘要

AbstractA reversed phase liquid chromatographic (RPLC) method was developed to simultaneously detect and quantify creatinine, quinolinic acid, gentisic acid and 4-hydroxybenzoic acid in urine. These four bio-markers are present in relatively high concentrations in urine. Using a 5% methanol in water mobile phase with 0.6% acetic acid and a Zorbax C18column, baseline resolution for all four biomarkers in synthetic urine was achieved. Better resolution was obtained for the separation of these four compounds when water rich mobile phases were used. Detection of the four biomarkers in urine using the proposed RPLC method is limited by background from the urine matrix for the later eluting compounds and from the dead marker for earlier eluting compounds.Highlights?This study demonstrates the advantages of using water-rich mobile phases in RPLC for biomarker analysis.?An inexpensive RPLC method has been developed to detect Renal Cell Carcinoma in its earlier stages.?Preliminary studies using synthetic urine are encour