An immunological determination of somatostatin in pharmaceutical by sandwich ELISA based on IgY and polyclonal antibody

摘要

A sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of Somatostatin based on double antibody sandwich method was studied in this paper. The Somatostatin specific Yolk antibody (IgY) and polyclonal antibody were available to Somatostatin-conjugated KLH immunized rabbits and chickens used to construct the sandwich ELISA for the measurement of Somatostatin. The limit of detection was 0.1 mu g/mL and the half maximal inhibitory concentration (IC50) was 0.859 pg/mL. The method developed in the study showed no evident cross-reactivity with other similar analogs. The recoveries of Somatostatin spiked mannitols were in the range of 98.45% to 103.90% with infra- and inter-day coefficient of variants (CVs) 8.71% and 4.60% respectively. The detection standard curve of Somatostatin exhibited a good linearity (R-2 = 0.9931, n = 3). Therefore, the methods could be selectively used for rapid screening of Somatostatin in pharmaceutical.