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PAMAM dendrimer modified screen printed electrodes for impedimetric detection of miRNA-34a

机译:PAMAM DENDIMER改进屏幕印刷电极,用于MIRNA-34A的阻碍检测

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In this study, the effective usage of PAMAM dendrimer was implemented for detection of microRNA-34a (miRNA-34a) which was known as a biomarker for many vital diseases such as cancer, Alzheimer, etc. The microscopic and electrochemical characterization of the PAMAM modified electrode surface was performed successively. The experimental conditions such as DNA probe, target concentration, hybridization time were optimized for the purpose of effective nucleic acid hybridization between miRNA-34a RNA target and its complementary DNA probe. Under optimum conditions, the selectivity of the impedimetric nucleic acid biosensor was tested in the presence of microRNA-15a (miRNA-15a) and microRNA-660 (miRNA-660). Our impedimetric biosensor platform could be selectively detect its target, miRNA-34a even if the hybridization was done in fetal bovine serum (FBS), which served a complex media for detection process. This disposable, easy-to-use, cheap and time saving impedimetric biosensor platform is the first study in the literature on behalf of electrochemical detection of miRNA-34a.
机译:在这项研究中,实施了PAMAM树枝状体的有效使用,用于检测MicroRNA-34a(miRNA-34a),其被称为癌症,阿尔茨海默等许多重要疾病的生物标志物。帕姆修饰的微观和电化学表征连续进行电极表面。在MiRNA-34A RNA靶标和其互补DNA探针之间的有效核酸杂交的目的,优化了DNA探针,靶浓度,杂交时间的实验条件。在最佳条件下,在MicroRNA-15a(miRNA-15a)和microRNA-660(miRNA-660)的存在下测试阻抗核酸生物传感器的选择性。即使在胎牛血清(FBS)中进行杂交,我们的阻抗生物传感器平台也可以选择性地检测其目标miRNA-34a,其为检测过程提供复杂的培养基。这种一次性,易于使用,便宜和节省的阻碍生物传感器平台是代表MiRNA-34a的电化学检测的文献中的第一次研究。

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