Rapid and sensitive extraction of aflatoxins by Fe3O4/zeolite nanocomposite adsorbent in rice samples

摘要

In this study, a magnetic nanocomposite was synthesized using a simple and efficient method, and its performance was evaluated through magnetic dispersive solid-phase extraction (MDSPE) of aflatoxins (AFs) in rice samples. The aqueous extract of Centaurea cyanus was used as a stabilizer and reducing agent in the synthesis of Fe3O4/zeolite nanocomposite. Field emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), Fourier-transform infrared spectroscopy (FT-IR), zeta potential, Brunauer-Emmett-Teller (BET), and vibrating sample magnetometer (VSM) were used to characterize the morphology of the adsorbent. The effects of nanocomposite dosage, vortex time, ionic strength, sample volume, and desorption conditions on the extraction efficiency of AFs were studied. The proposed nanocomposite showed excellent binding properties to AFs in the range of 2-10 ng g(-1), and 82-96% of AFs were extracted within the first minute of contact. The limits of detection (LODs) were 0.1 and 0.02 ng g(-1) for AFB1-AFG1 and AFB2-AFG2, respectively. Moreover, the limits of quantification (LOQs), were 0.4 and 0.08 ng g(-1) for AFB1-AFG1 and AFB2-AFG2, respectively. Compared to other methods for the extraction and determination of AFs, the suggested MDSPE-LC/FD method possessed advantages such as rapidity, simplicity, excellent figures of merit, and ecofriendly nature.