Antimicrobial Activity of Prodigiosin from Serratia sp. PDGS~(120915) Against Intestinal Pathogenic Bacteria

摘要

This study aimed to identify and characterize the antimicrobial activity of prodigiosin produced by Serratia sp. PDGS120915 isolated from stream water in Busan, Korea; the identification was performed using phonological, biochemical, and molecular techniques, including 16S rRNA sequence analysis. Prodigiosin from the bacterial culture was purified by high-performance liquid chromatography (HPLC), and its antimicrobial activity and minimum inhibitory concentrations (MICs) were evaluated against 10 intestinal pathogenic gram-positive and negative bacteria. The results revealed that the isolated prodigiosin exhibited high antimicrobial activity against Listeria monocytogenes, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, and Vibrio parahaemolyticus; further, the isolated prodigiosin showed minimum inhibitory concentrations (MICs) between 3 ug/ml and 30 mg/ml, but they were not active against Bacillus subtilis, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli. In conclusion, prodigiosin isolated from Serratia sp. PDGS120915 showed high antimicrobial activity against intestinal pathogenic bacteria and has potential applications in the development of new antimicrobial agents.