A Study on the Whitening Effect of Erigeron annuus (L.) Pers. Ethanoi Extract on Melanoma Cell (B16F10)

摘要

A 70% ethanoi extract of Erigeron annuus (L.) Pers. was investigated for its whitening activity for application as a functional ingredient in cosmetic products. At the E. annuus extract concentration of 1,000 ug/ml, the electron-donating ability was found to be 67.83%, the tyrosinase inhibitory effect (related to skin-whitening) was 69%, the elastase inhibitory effect (related to skin-wrinkling) was 69%, and the astringent effect was 80%. The ABTS+ radical-scavenging ability was 87% at the 500 u,g/mlconcentration. In the cell viability test measured on melanoma cells, 96% of the cells treated with 100 ug/ml of the extract were viable. According to the western blot results, the protein expression of the microphthalmia-associated transcription factor(MITF), tyrosinase, tyrosinase-related protein (TRP) -1, and TRP-2 was decreased by 60.22%, 47.83%, 54.79%, and 67.88%, respectively, at the extract concentration of 100 pg/ml. The protein expression of phos-phorylated extracellular signal regulated kinase (p-ERK) and phosphorylated cAMP response element-binding protein (p-CREB) was decreased with increasing concentrations of the extract. Reverse transcrip-tion-polymerase chain reaction of the extract showed that the mRNA expression of MITF, tyrosinase, TRP-1, and TRP-2 was decreased by 86.51%, 85.22%, 74.26%, and 66.66%, respectively, at 100 ug/ml extract concentration. The findings suggest that the 70% ethanoi extract from E. annuus (L.) Pers. has potential as a cos-meceutical ingredient with whitening effect.