An Overview of data science uses in bioimage informatics

摘要

Highlights ? A practical overview of data science techniques as used in bioimage informatics. ? Look at answers to the question ‘my images are segmented, now what?’. ? Aimed at biologist with an interest in computational techniques. Abstract This review aims at providing a practical overview of the use of statistical features and associated data science methods in bioimage informatics. To achieve a quantitative link between images and biological concepts, one typically replaces an object coming from an image (a segmented cell or intracellular object, a pattern of expression or localisation, even a whole image) by a vector of numbers. They range from carefully crafted biologically relevant measurements to features learnt through deep neural networks. This replacement allows for the use of practical algorithms for visualisation, comparison and inference, such as the ones from machine learning or multivariate statistics. While originating mainly, for biology, in high content screening, those methods are integral to the use of data science for the quantitative analysis of microscopy images to gain biological insight, and they are sure to gather more interest as the need to make sense of the increasing amount of acquired imaging data grows more pressing.