Dose-response Effect of Glutathione added in Parenteral Nutrition on Lung Oxidative Stress and Alveolarization in Newborn Guinea Pig

摘要

Peroxides contaminating parenteral nutrition (PN) are associated with oxidized redox potential of glutathione in blood of preterm newborns ( Methods At day 3 of life, through a jugular catheter, guinea pigs (N=55) received PN (dextrose, amino acids, lipids, vitamins, electrolytes) enriched in glutathione (0, 50, 120, 165, 270, 370, 650 μg GSSG/kg/d). A control group (no manipulation, fed by mouth) served as reference. After 4 days, lungs were taken for determination of GSH, GSSG, redox potential (capillary electrophoresis) and alveolarization index (AI) (number of intercepts between a 1 mm line and histological structures). Data (mean ± sem) from animals on PN were compared by ANOVA, p Results The low AI observed with low doses (μg/kg/d) of GSSG (0-120: 26±1) was corrected with higher level (165-650: 30±1, p Conclusion Addition of glutathione into PN allows detoxification of peroxides (lower GSSG), preventing oxidation of redox and loss of alveoli. Our team is funded to start a clinical study soon.