Crystallization and preliminary X-ray crystallographic analysis of alginate lyase Al-II from Sphingomonas species A1

摘要

Alginate lyase Al-II of Sphingomonas species A1 was purified and crystallized using the hanging drop vapor-diffusion method in 0.1 M Tris-HCl buffer containing 43% saturated ammonium sulfate, 8% polyethylene glycol 4000 and 0.2 M Li_2SO_4 at pH 8.5 and 20℃. The crystals are tetragonal and belong to the space group P4_32_12 or P4_12_12 with unit cell dimensions of a = b = 144.07 and c = 296.38 A. The diffraction data up to 2.8 A were collected by a synchrotron radiation source at SPring-8 in Japan.