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Molecular Identification and Comparative Evaluation of Tropical Marine Microalgae for Biodiesel Production

机译:热带海洋微藻对生物柴油生产的分子鉴定及比较评价

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Marine microalgae have emerged as important feedstock for liquid biofuel production. The identification of lipid-rich native microalgal species with high growth rate and optimal fatty acid profile and biodiesel properties is the most challenging step in microalgae-based biodiesel production. In this study, attempts have been made to bio-prospect the biodiesel production potential of marine and brackish water microalgal isolates from the west coast of India. A total of 14 microalgal species were isolated, identified using specific molecular markers and based on the lipid content; seven species with total lipid content above 20% of dry cell weight were selected for assessing biodiesel production potential in terms of lipid and biomass productivities, nile red fluorescence, fatty acid profile and biodiesel properties. On comparative analysis, the diatoms were proven to be promising based on the overall desirable properties for biodiesel production. The most potential strain Navicula phyllepta MACC8 with a total lipid content of 26.54 % of dry weight of biomass, the highest growth rate (0.58 day(-1)) and lipid and biomass productivities of 114 and 431 mgL(-1) day(-1), respectively, was rich in fatty acids mainly of C16:0, C16:1 and C18:0 in the neutral lipid fraction, the most favoured fatty acids for ideal biodiesel properties. The biodiesel properties met the requirements of fuel quality standards based on empirical estimation. The marine diatoms hold a great promise as feedstock for large-scale biodiesel production along with valuable by-products in a biorefinery perspective, after augmenting lipid and biomass production through biochemical and genetic engineering approaches.
机译:海洋微藻已成为液体生物燃料生产的重要原料。具有高生长速率和最佳脂肪酸型材和生物柴油属性的富含脂质的天然微藻物种是微藻基生物柴油生产中最具挑战性的步骤。在这项研究中,已经对来自印度西海岸的海洋和咸水微藻分离株的生物柴油生产潜力进行了生物展望。使用特定分子标记物鉴定出共14种微藻物种,并基于脂质含量鉴定;选择七种具有高于20%的干细胞重量的七种,用于评估脂质和生物质生产率,尼罗红荧光,脂肪酸谱和生物柴油性能方面的生物柴油生产潜力。在比较分析上,证明硅藻基于生物柴油生产的总体理想性质,是有前途的。最潜在的菌株Navicula Phyllepta Macc8,总脂质含量为生物质的干重的26.54%,生长率最高(0.58天(-1))和114和431mg1(-1)天( - - 1)分别富含脂肪酸,主要是C16:0,C16:1和C18:0在中性脂质级分中,最有利的脂肪酸用于理想的生物柴油。生物柴油属性基于实证估计达到燃料质量标准的要求。海洋硅藻作为大规模生物柴油生产的原料,以及通过生物化学和基因工程方法增强脂质和生物量产量的生物遗产透视中的大型生物柴油生产以及有价值的副产品。

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