Effect of Bovine Serum Albumin on Red Blood Cell Optical Anisotropy Probed Through the Optomechanical Response in an Optical Trap

摘要

>The dynamics of trapped entities in an Optical Trap (OT) can yield information with regards to their viscoelastic response as well as optical anisotropy, if any. Detailed analysis of such dynamics correlated with parameters which affect the response can yield additional clues to the exact effect of these on the trapped entities. In this work, we illustrate this point by showing how the altered behavior of Red Blood Cells (RBC) treated with Bovine Serum Albumin (BSA) yields information about the nature of action of BSA, on which there is no current consensus in literature. We conclude from our studies that BSA treatment leads to a change in the birefringence of the RBCs, a conclusion arrived at from the altered optomechanical response of such cells in a linearly polarized Gaussian beam OT. Furthermore, we argue that the observed changes in cellular optical anisotropy may be thought of as due to changes in the curvature of the RBC membrane. We also note that BSA action could help mimic pathological conditions that result in an altered cell shape.