INTERACTION OF THE FUNGICIDE TEBUCONAZOLE WITH HUMAN SERUM ALBUMIN: A PRELIMINARY STUDY

摘要

The interactions between the fungicide tebucon-azole and human serum albumin were investigated using fluorescence and circular dichroism spectroscopies. The experimental results showed that the fluorescence quenching of the protein by the tebuconazolemolecule was a result of the formation of a ligand-protein complex with a binding constant of 8.51 x 103 l.mol-1 and the number of binding sites in the macro molecule was close to 1. These findings demonstrated the fact that although the binding affinity of tebuconazole to the protein may be slight, it was very similar to other triazole fungicides. In addition, tebuconazole stabilized the a-helical secondary structure of the human serum albumin due to the increase of the a con tent in the protein macromolecule.