ASSESSMENT OF POTENTIAL CLASTOGENIC EFFECT OF THE INSECTICIDE MOSPILAN8 20SP IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES AFTER IN VITRO EXPOSURE

摘要

Acetamiprid, that is known as the commercial formulation Mospilan* 20SP is the part of the neonicotinoid insecticide group and is widely used against various pests. In our study we assessed the potential clastogenic effects of Mospilan* in human peripheral blood lymphocytes in vitro using a chromosome aberration test. The lymphocytes were treated with acetamiprid in the concentration range of 5,10,25 and 50 ug.ml1 for 24 and 48h. After 24h exposure, the insecticide induced statistically significant higher levels of chromosome aberrations from the concentration of lOug.ml"1 (P<0.05 and P<0.001) and a significant decrease in mitotic index (MI) at the concentrations of 25 and 50 ug.ml1 (P<0.05 and P<0.01), respectively. After a 48 h exposure, we founda dose dependent increase in the percentage of chromosome aberrations at all concentrations (P<0.05; P<0.01 and P< 0.001) and a decrease in MI at concentrations of 25 and 50 ug.mk1 (P < 0.05 and P < 0.01). Our results indicated that neonicotinoid insecticide formulations containing acetamiprid may have potential cytotoxic and genotoxic effects.