Application of High-Performance Liquid Chromatography with Diode Array Detector for Simultaneous Determination of 11 Synthetic Dyes in Selected Beverages and Foodstuffs

摘要

A simple, inexpensive and robust high-performance liquid chromatography diode array detector (HPC-DAD) procedures are proposed to analyse food dyes in beverages, hard candy and fish roe samples. An ether-linked phenyl stationary phase provides sufficient selectivity and chromatographic performance for separation of 11 sulfonated azo dyes. Beverage samples were only diluted (and degassed when needed) before analysis. Solid-phase extraction (SPE) or matrix solid-phase dispersion (MSPD) procedures are proposed for efficient extraction of the analytes from candies or fish roe samples, respectively. Limits of detection (LODs) were from 0.005 to 0.013 mu g mL(-1) and limits of quantification (LOQs) between 0.014 and 0.038 mu g mL(-1). HPLC-DAD method was validated in terms of intra- and inter-day accuracy and precision at three concentration levels 2, 1, and 0.1 mu g mL(-1). Validation was also performed for SPE and MSPD extraction procedures including intra- and inter-day accuracy (Recovery %) and precision (RSD%), as well as intra-laboratory reproducibility. Application to analysis of beverages and food samples available to consumers proved that described methods are suitable for the routine analysis of dyes in food products.