Stacked graphene-Al _2O _3 nanopore sensors for sensitive detection of DNA and DNA-protein complexes

摘要

We report the development of a multilayered graphene-Al _2O _3 nanopore platform for the sensitive detection of DNA and DNA-protein complexes. Graphene-Al _2O _3 nanolaminate membranes are formed by sequentially depositing layers of graphene and Al _2O _3, with nanopores being formed in these membranes using an electron-beam sculpting process. The resulting nanopores are highly robust, exhibit low electrical noise (significantly lower than nanopores in pure graphene), are highly sensitive to electrolyte pH at low KCl concentrations (attributed to the high buffer capacity of Al _2O _3), and permit the electrical biasing of the embedded graphene electrode, thereby allowing for three terminal nanopore measurements. In proof-of-principle biomolecule sensing experiments, the folded and unfolded transport of single DNA molecules and RecA-coated DNA complexes could be discerned with high temporal resolution. The process described here also enables nanopore integration with new graphene-based structures, including nanoribbons and nanogaps, for single-molecule DNA sequencing and medical diagnostic applications.