Evaluation of Antiradical and Anti-Inflammatory Activities of Ethyl Acetate and Butanolic Subfractions of Agelanthus dodoneifolius (DC.) Polhill & Wiens (Loranthaceae) Using Equine Myeloperoxidase and Both PMA-Activated Neutrophils and HL-60 Cells

摘要

The ethyl acetate and n-butanolic subtractions of Agelanthus dodoneOlius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorolluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total N4P0 release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subtraction, at least in respect of the chemiluminescence test (IC50 = 0.3 +/- 0.1 mu g/mL) and the ELBA and SIFTED assays (IC50 = 2.8 +/- 1.2 mu g/mt, and 1.3 +/- 1.0 mu g/mL), respectively. However, the ethyl acetate subtraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subtraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subtraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dothmetfolius subtractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds.