Expression profile of IL-1 family cytokines in aqueous humor and sera of patients with HLA-B27 associated anterior uveitis and idiopathic anterior uveitis

摘要

The purpose of this study was to evaluate the cytokine expression profile of specific IL-1 family members in the aqueous humor and sera of patients with HLA-B27 associated acute anterior uveitis (MU) and idiopathic MU. Following informed consent, a total of 13 patients with HLA-B27 associated AAU, 12 patients with idiopathic MU and 9 controls were recruited to this study from May 2013 to July 2014. Each individual received a complete ophthalmologic examination. Aqueous humor and sera samples were collected and 11 inflammation-related cytokines of the IL-1 family (IL-1 alpha, IL-1 beta, IL-1 receptor antagonist [IL-1Ra], IL-18, IL-36 receptor antagonist [IL-36Ra], IL-33, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-37, IL-38) were quantitatively measured and analyzed for statistical significance between groups. The degree of inflammation, anterior chamber cell or flare, correlated with expression of IL-1 beta, IL-1Ra, and IL-18. The highest levels of IL-1 beta, IL-1Ra, IL-18, and IL-36Ra were seen in the aqueous of patients with HLA-B27 associated AAU and this was statically significant when compared to the controls, but not to idiopathic MU. Expression of IL-18 was statistically higher in the aqueous of patients with HLA-B27 associated MU in comparison to either idiopathic MU or controls, but this may reflect greater inflammation in this patient group. In the sera only IL-1 alpha was statistically higher in the HLA-B27 associated MU in comparison to the control.