Topical silver and gold nanoparticles complexed with Cornus mas Cornus mas suppress inflammation in human psoriasis plaques by inhibiting NF NF ‐κB activity

摘要

Abstract New biomaterials based on nanoparticles ( NP s) carrying polyphenols‐rich extracts ( Cornus mas) recently showed promising anti‐inflammatory activity in psoriasis. We aimed to understand how topically delivered silver and gold nanoparticles complexed with Cornus mas (Ag‐ NP s‐ CM , Au‐ NP s‐ CM ) modulate inflammation in psoriasis at cellular and molecular level. The impact on psoriatic inflammation was assessed in vitro on pro‐inflammatory macrophages, by clinical score, high‐frequency ultrasonography and immunohistology of psoriasis plaques treated with Ag‐ NP s‐ CM , Au‐ NP s‐ CM or control. Incubation of pro‐inflammatory macrophages with nanoparticles significantly decreased the release of NO , IL ‐12 and TNF ‐α. Immunofluorescence confirmed that nanoparticles significantly reduced CD 68‐positive macrophages and their IL ‐12 and TNF ‐α production in human psoriasis plaques. NP s‐ CM appear to repress NF ‐κB activation in macrophages, inhibiting the production of pro‐inflammatory factors with causal role in psoriasis. Ag and Au NP s‐ CM represent a novel nanoparticle‐based “green” technology which may provide an efficient tool for modern psoriasis therapy, circumventing immunosuppression‐related side effects of biologicals.