Evolution of correlation between Helicobacter pylori infection and autoimmune liver disease

摘要

The present study was planned to evaluate correlation between Helicobacter pylori(HP) infection and autoimmune liver disease(AILD). A total of 60 patients diagnosed with AILD in Affiliated Hospital of Binzhou Medical College were continuously enrolled in the present study. HP infection was detected by 13C-urea breath test. The levels of anti-myeloperoxidase were tested by ELISA. The positive rate of anti-nuclear antibody(ANA), anti-mitochondrial antibody(AMA), anti-smooth muscle antibody(SMA) and anti-neutrophil cytoplasm antibody(ANCA) were tested by indirect immunofluorescence. The positive rates of anti-mitochondrial antibody(AMA-M2), anti-liver-kidney microsomal antibody(LKM-1), anti-liver cytoplasm anti-body I( LC-1) and anti-soluble liver antigen/liver-pancreas antigen(SLA/LP) were tested by immunoblotting. Liver function indexes including alanine transaminase, aspartate transaminase, alkaline phosphatase and glutamyltransferase, were analyzed with a fully automatic biochemical analyzer. The levels of serum cytokine IFN-gamma, interleukin-6(IL-6), IL-10 and tumor necrosis factor-alpha (TNF-alpha) were tested by ELISA. A total of 37 patients(61.67%) were observed to be HP-positive. MPO-positive rate, positive rate of ANA, AMA, SMA and ANCA and positive rate of AMA-M2, LKM-1, LC-1 and SLA/LP in patients with positive HP infection were significantly higher than those of patients with negative HP infection. On the other hand, the levels of liver function indices did not showed any significant differences among HP-positive cases or HP-negative cases. However, the levels wof IFN-gamma, IL-6, IL-10 and TNF-alpha in patients with positive HP infection were significantly higher than those of patients with negative HP infection. In conclusion, the positive infection rate of HP infection in patients with AILD is high and is closely associated with various positive immune antibodies as well as cytokine levels.