Autophagy‐related gene        <i xmlns='http://www.wiley.com/namespaces/wiley'>BbATG11BbATG11        is indispensable for pexophagy and mitophagy, and contributes to stress response, conidiation and virulence in the insect mycopathogen        <i xmlns='http://www.wiley.com/namespaces/wiley'>Beauveria bassianaBeauveria bassiana

Ding Jin‐Li; Peng Yue‐Jin; Chu Xin‐Ling; Feng Ming‐Guang; Ying Sheng‐Hua

摘要

Summary > Autophagy is a conserved degradation system in eukaryotic cells that includes non‐selective and selective processes. Selective autophagy functions as a selective degradation mechanism for specific substrates in which autophagy‐related protein 11 (ATG11) acts as an essential scaffold protein. In B. bassiana , there is a unique ATG11 family protein, which is designated as BbATG11 . Disruption of BbATG11 resulted in significantly reduced conidial germination under starvation stress. The mutant Δ BbATG11 displayed enhanced sensitivity to oxidative stress and impaired asexual reproduction. The conidial yield was reduced by approximately 75%, and this defective phenotype could be repressed by increasing exogenous nutrients. The virulence of the Δ BbATG11 mutant strain was significantly impaired as indicated in topical and intra‐hemocoel injection bioassays, with a greater reduction in topical infection. Notably, BbATG11 was involved in pexophagy and mitophagy, but these two autophagic processes appeared in different fungal physiological aspects. Both pexophagy and mitophagy were associated with nutrient shift, starvation stress and growth in the host hemocoel, but only pexophagy appeared in both oxidation‐stressed cells and aerial mycelia. This study highlights that BbATG11 mediates pexophagy and mitophagy in B. bassiana and links selective autophagy to the fungal stress response, conidiation and virulence. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >自噬是一种保守的降解系统，其包括非选择性和选择性过程的真核细胞。选择性自噬作用作为特定底物的选择性降解机制，其中自噬相关蛋白11（ATG11）作为必要的支架蛋白。在 b。 Bassiana </ i>，有一个独特的ATG11家族蛋白质，被指定为 BBATG11 </ i>。 Bbatg11的破坏导致饥饿胁迫下显着降低的分析萌发。突变体δ BBATG11 </ i>显示出对氧化应激的敏感性和无性繁殖受损。结合产率降低约75％，并且可以通过增加外源营养物来抑制这种有缺陷的表型。如局部和血管内注射生物测定在局部和血管内注射生物测定中所示，δ2BBATG11</ i>突变菌株的致毒力显着损害，局部感染较大。值得注意的是， Bbatg11 </ i>涉及磷灰和乳化物，但这两种自噬过程出现在不同的真菌生理方面。 Pexophagy和MINOCHAGY都与营养换档，饥饿胁迫和生长相关，但仅在氧化胁迫细胞和空中菌丝体中出现百分比。这项研究突出显示 Bbatg11 </ i>在 b中介导pexophagy和mitophy。 Bassiana </ i>并将选择性自噬与真菌应激反应，结合和毒力联系起来。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第9期</span><b style="margin: 0 2px;">|</b><span>共16页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ding Jin‐Li&option=202" target="_blank" rel="nofollow">Ding Jin‐Li;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peng Yue‐Jin&option=202" target="_blank" rel="nofollow">Peng Yue‐Jin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chu Xin‐Ling&option=202" target="_blank" rel="nofollow">Chu Xin‐Ling;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Feng Ming‐Guang&option=202" target="_blank" rel="nofollow">Feng Ming‐Guang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ying Sheng‐Hua&option=202" target="_blank" rel="nofollow">Ying Sheng‐Hua;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Microbiology College of Life SciencesZhejiang UniversityHangzhou 310058 China;</p> <p>Institute of Microbiology College of Life SciencesZhejiang UniversityHangzhou 310058 China;</p> <p>Institute of Microbiology College of Life SciencesZhejiang UniversityHangzhou 310058 China;</p> <p>Institute of Microbiology College of Life SciencesZhejiang UniversityHangzhou 310058 China;</p> <p>Institute of Microbiology College of Life SciencesZhejiang UniversityHangzhou 310058 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229656.html">Autophagy‐related gene <i xmlns="http://www.wiley.com/namespaces/wiley">BbATG11BbATG11 is indispensable for pexophagy and mitophagy, and contributes to stress response, conidiation 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Autophagy‐related gene BbATG11BbATG11 is indispensable for pexophagy and mitophagy, and contributes to stress response, conidiation and virulence in the insect mycopathogen Beauveria bassianaBeauveria bassiana

摘要