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首页> 外文期刊>Environmental microbiology >Diversity, specificity and molecular evolution of the lytic arsenal of PseudomonasPseudomonas phages: in silicoin silico perspective
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Diversity, specificity and molecular evolution of the lytic arsenal of PseudomonasPseudomonas phages: in silicoin silico perspective

机译：假单胞菌的裂变，特异性和分子演变，硅藻土的裂解，硅噬菌体噬菌体（Silico）在硅的角度下

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Summary > Bacteriophages encode an arsenal of proteins to lyse bacteria by breaking their surface structures, constituting a promising alternative to antibiotics. However, the selection and bioengineering of endolysins and other phage lytic proteins need to be assisted by a previous knowledge of their molecular characteristics. In this study, all putative lytic proteins encoded in Pseudomonas phages were in silico examined to describe their diversity, host association and molecular evolution. A total of 491 proteins were identified among 223 phages, including endolysins, holins, pinholins, spanins, lipases and peptidases. Protein families and combination of functional domains were characteristic of phages belonging to the same genus, and these tended to infect a single host species. Clustering and phylogenetic analysis showed a protein grouping associated with bacterial host, and some functional domains being specific. Interestingly, most putative lytic proteins from phages infecting P. fluorescens and P. putida had negative net charges, opposed to most endolysins. Phage lifestyle also had an impact on protein variability, with transglycosylases, glucosaminidases, holins and spanins from lysogenic phages clustering into monophyletic nodes, suggesting the effect of a different selection pressure as a result of the co‐option of a new function in the lysogenized bacteria. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>概述</标题> >通过破坏其表面结构，构成有希望的替代方案，将蛋白质的蛋白质编码为粘附的细菌。抗生素。然而，通过先前的分子特征的了解，需要通过先前的分子特性的知识来辅助子溶解和其他噬菌体裂解蛋白的选择和生物工程。在这项研究中，在硅藻中编码的所有推定的裂解蛋白在硅中均为I>以描述其多样性，宿主协会和分子演进。在223个噬菌体中鉴定了总共491个蛋白质，包括卵溶蛋白，孔，澄清温，辛酸，脂肪酶和肽酶。蛋白质家族和功能性域的组合是属于同一属的噬菌体的特征，这些趋势是感染单一宿主物种。聚类和系统发育分析显示与细菌宿主相关的蛋白质分组，以及一些功能域特异性。有趣的是，来自噬菌体的大多数推定的裂解蛋白感染 p。荧光荧光荧光笔</ i> p。 Pieida </ i>具有负净充电，而不是大多数indolysins。噬菌体生活方式也对蛋白质变异性产生了影响，用羟基糖基酶，葡糖胺酶，血清素，从溶血基噬菌体聚集成单胞节节点，表明不同选择压力的效果是溶酶化细菌中的新功能的共选项的结果。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology》</a> <b style="margin: 0 2px;">|</b><span>2019年第11期</span><b style="margin: 0 2px;">|</b><span>共15页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Valero‐Rello Ana&option=202" target="_blank" rel="nofollow">Valero‐Rello Ana;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Independent ResearcherValencia 46009 Spain;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229633.html">Diversity, specificity and molecular evolution of the lytic arsenal of <i >PseudomonasPseudomonas phages: <i >in silicoin silico perspective</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Valero‐Rello Ana&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Valero‐Rello Ana </a> <a href="/journal-foreign-22868/" target="_blank" rel="nofollow" class="tuijian_authcolor">Environmental microbiology .</a> <span>2019</span><span>,第11期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：假单胞菌的裂变，特异性和分子演变，硅藻土的裂解，硅噬菌体噬菌体（Silico）在硅的角度下</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023986068.html">Simeprevir oxidative degradation product: Molecular modeling, <i >in silicoin silico toxicity and resolution by synchronous spectrofluorimetry</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Attia Khalid A. 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