Comparative proteome analysis of propionate degradation by <i xmlns='http://www.wiley.com/namespaces/wiley'>Syntrophobacter fumaroxidansSyntrophobacter fumaroxidans in pure culture and in coculture with methanogens

Sedano‐Nú?ez Vicente T.; Boeren Sjef; Stams Alfons J. M.; Plugge Caroline M.

摘要

Summary > Syntrophobacter fumaroxidans is a sulfate‐reducing bacterium able to grow on propionate axenically or in syntrophic interaction with methanogens or other sulfate‐reducing bacteria. We performed a proteome analysis of S. fumaroxidans growing with propionate axenically with sulfate or fumarate, and in syntrophy with Methanospirillum hungatei , Methanobacterium formicicum or Desulfovibrio desulfuricans . Special attention was put on the role of hydrogen and formate in interspecies electron transfer (IET) and energy conservation. Formate dehydrogenase Fdh1 and hydrogenase Hox were the main confurcating enzymes used for energy conservation. In the periplasm, Fdh2 and hydrogenase Hyn play an important role in reverse electron transport associated with succinate oxidation. Periplasmic Fdh3 and Fdh5 were involved in IET. The sulfate reduction pathway was poorly regulated and many enzymes associated with sulfate reduction (Sat, HppA, AprAB, DsrAB and DsrC) were abundant even at conditions where sulfate was not present. Proteins similar to heterodisulfide reductases (Hdr) were abundant. Hdr/Flox was detected in all conditions while HdrABC/HdrL was exclusively detected when sulfate was available; these complexes most likely confurcate electrons. Our results suggest that S. fumaroxidans mainly used formate for electron release and that different confurcating mechanisms were used in its sulfidogenic metabolism. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> > <i > Syntrophocacter Fumaroxidans </ i>是一种硫酸盐还原细菌，能够在丙酸盐上或与甲烷或其他硫酸盐还原细菌的同步相互作用上生长。我们对进行了蛋白质组分析。 Fumaroxidans </ i>与硫酸盐或富马酸盐保持着丙酸盐，并在与甲基汞Hungatei ，甲基杆菌甲醛，或脱硫脱硫脱硫剂</ i>。特别关注氢气的作用，甲酸在梭坡中的作用和甲酸中的含量电子转移（IET）和节能。甲酸脱氢酶FDH1和氢酶HOX是用于节能的主要诱变酶。在周质中，FDH2和氢化酶HyN在与琥珀酸氧化相关的反向电子传输中起重要作用。周质FDH3和FDH5参与IET。硫酸盐还原途径较差，即使在不存在硫酸盐的条件下，即使在不存在硫酸盐的条件下也具有许多与硫酸盐还原（SAT，HPPA，APRAB，DSRAb和DSRC）的酶。类似于异代硫化还原酶（HDR）的蛋白质丰富。在所有条件下检测到HDR / FLOX，同时硫酸盐可用时完全检测到HDRABC / HDR1;这些配合物最有可能互动电子。我们的结果表明 s。 Fumaroxidans </ i>主要用于电子释放的甲酸盐，并且在其亚硫化代谢中使用不同的互动机制。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共15页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sedano‐Nú?ez Vicente T.&option=202" target="_blank" rel="nofollow">Sedano‐Nú?ez Vicente T.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Boeren Sjef&option=202" target="_blank" rel="nofollow">Boeren Sjef;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Stams Alfons J. M.&option=202" target="_blank" rel="nofollow">Stams Alfons J. M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Plugge Caroline M.&option=202" target="_blank" rel="nofollow">Plugge Caroline M.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Laboratory of MicrobiologyWageningen University &</p> <p>Research Stippeneng 4Wageningen The Netherlands;</p> <p>Laboratory of BiochemistryWageningen University &</p> <p>Research Stippeneng 4Wageningen The Netherlands;</p> <p>Laboratory of MicrobiologyWageningen University &</p> <p>Research Stippeneng 4Wageningen The Netherlands;</p> <p>Laboratory of MicrobiologyWageningen University &</p> <p>Research Stippeneng 4Wageningen The Netherlands;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229341.html">Comparative proteome analysis of propionate degradation by <i xmlns="http://www.wiley.com/namespaces/wiley">Syntrophobacter fumaroxidansSyntrophobacter fumaroxidans in pure culture and in coculture with methanogens</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sedano‐Nú?ez Vicente T.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sedano‐Nú?ez Vicente T.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Boeren Sjef&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Boeren Sjef,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Stams Alfons J. 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Comparative proteome analysis of propionate degradation by Syntrophobacter fumaroxidansSyntrophobacter fumaroxidans in pure culture and in coculture with methanogens

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