A two‐step evolutionary process establishes a non‐native vitamin B6 pathway in <i xmlns='http://www.wiley.com/namespaces/wiley'>Bacillus subtilisBacillus subtilis

Rosenberg Jonathan; Yeak KahYen C.; Commichau Fabian M.

摘要

Summary > Pyridoxal 5′‐phosphate (PLP), the most important form of vitamin B6 serves as a cofactor for many proteins. Two alternative pathways for de novo PLP biosynthesis are known: the short deoxy‐xylulose‐5‐phosphate (DXP)‐independent pathway, which is present in the Gram‐positive model bacterium Bacillus subtilis and the longer DXP‐dependent pathway, which has been intensively studied in the Gram‐negative model bacterium Escherichia coli . Previous studies revealed that bacteria contain many promiscuous enzymes causing a so‐called ‘underground metabolism’, which can be important for the evolution of novel pathways. Here, we evaluated the potential of B. subtilis to use a truncated non‐native DXP‐dependent PLP pathway from E. coli for PLP synthesis. Adaptive laboratory evolution experiments revealed that two non‐native enzymes catalysing the last steps of the DXP‐dependent PLP pathway and two genomic alterations are sufficient to allow growth of vitamin B6 auxotrophic bacteria as rapid as the wild type. Thus, the existence of an underground metabolism in B. subtilis facilitates the generation of a pathway for synthesis of PLP using parts of a non‐native vitamin B6 pathway. The introduction of non‐native enzymes into a metabolic network and rewiring of native metabolism could be helpful to generate pathways that might be optimized for producing valuable substances. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >吡哆aral 5 ' - 磷酸盐（PLP），最重要的维生素B6形式用作许多蛋白质的辅因子。用于 de novo-i> PLP生物合成的两种替代途径是已知的：短脱氧 - 木糖糖-5-磷酸（DXP） - 依赖性途径，其存在于革兰氏阳性模型细菌中枯草芽孢杆菌< / i>和较长的DXP依赖性途径，在革兰氏阴性模型细菌中被密集地研究了大肠杆菌菌。以前的研究表明，细菌含有许多混杂的酶，导致所谓的“地下代谢”，这对于新途径的演变可能是重要的。在这里，我们评估了 b的潜力。 suptilis </ i>从 e使用截断的非本机DXP相关的PLP路径。 Coli </ i>用于PLP合成。自适应实验室演化实验表明，两种非天然酶催化DXP依赖性PLP途径的最后步骤和两个基因组改变是足以允许维生素B6滋巢细菌的生长作为野生型。因此，在 b中存在地下代谢。枯草芽孢杆菌利用非本地维生素B6途径的部分促进用于合成PLP的途径。将非原生酶引入代谢网络和本地代谢的重新灌注可能有助于产生可能优化用于生产有价值物质的途径。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第1期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rosenberg Jonathan&option=202" target="_blank" rel="nofollow">Rosenberg Jonathan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yeak KahYen C.&option=202" target="_blank" rel="nofollow">Yeak KahYen C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Commichau Fabian M.&option=202" target="_blank" rel="nofollow">Commichau Fabian M.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of General MicrobiologyInstitute for Microbiology and Genetics Georg‐August‐University G?ttingenG?ttingen Germany;</p> <p>Department of General MicrobiologyInstitute for Microbiology and Genetics Georg‐August‐University G?ttingenG?ttingen Germany;</p> <p>Department of General MicrobiologyInstitute for Microbiology and Genetics Georg‐August‐University G?ttingenG?ttingen Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul 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纪念中国微生物学会成立六十周年大会暨2012年中国微生物学会学术年会 </a> <span> <span> . 2012</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314041726.html">土壤中枯草芽孢杆菌（Bacillus subtilis）B908的分子检测</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李扬苹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李扬苹</a> <span> . 2004</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130400748792.html">B6 COMPOSITION FOR PREVENTING IMPROVING OR TREATING SLEEP DISORDERS COMPRISING FERMENTATION BROTH OF BACILLUS SUBTILIS AND VITAMIN B6 AS AN EFFECTIVE INGREDIENT</a> <b>[P]</b> . <span> 外国专利：  KR20200043250A </span> <span> . 2020-04-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：B6用于预防或改善睡眠障碍的组合物，其包括芽孢杆菌和维生素B6的发酵液作为有效成分 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130400579716.html">B6 COMPOSITION FOR PREVENTING IMPROVING OR TREATING SLEEP DISORDERS COMPRISING FERMENTATION BROTH OF BACILLUS SUBTILIS AND VITAMIN B6 AS AN EFFECTIVE INGREDIENT</a> <b>[P]</b> . <span> 外国专利：  KR102149404B1 </span> <span> . 2020-08-31</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：B6用于预防或改善睡眠障碍的组合物，其包括芽孢杆菌和维生素B6的发酵液作为有效成分 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" 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A two‐step evolutionary process establishes a non‐native vitamin B6 pathway in Bacillus subtilisBacillus subtilis

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