Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen <i xmlns='http://www.wiley.com/namespaces/wiley'>Vibrio alginolyticusVibrio alginolyticus

Yang Zhen; Zhou Xiaohui; Ma Yue; Zhou Mian; Waldor Matthew K.; Zhang Yuanxing; Wang Qiyao

摘要

Summary > Type VI secretion systems (T6SS) are multiprotein secretion machines that can mediate killing of bacterial cells and thereby modify the composition of bacterial communities. The mechanisms that control the production of and secretion of these killing machines are incompletely understood, although quorum sensing (QS) and the PpkA kinase modulate T6SS activity in some organisms. Here we investigated control the T6S in the marine organism Vibrio alginolyticus EPGS, which encodes two T6SS systems (T6SS1 and T6SS2). We found that the organism principally relies on T6SS2 for interbacterial competition. We further carried out a phosphoproteomic screen to identify substrates of the T6SS2‐linked PpkA2 kinase. Substrates of PpkA2 encoded within the T6SS2 cluster as well proteins that are apparently not linked to T6SS‐related processes were identified. Similar to other organisms, PpkA2 autophosphorylation was critical for T6SS2 function. Notably, phosphorylation of a polypeptide encoded outside of the T6SS2 cluster, VtsR, was critical for T6SS2 expression and function because it augments the expression of luxR , a key regulator of QS that also promotes T6SS2 gene expression. Thus, PpkA2 controls a phosphorylation cascade that mediates a positive regulatory loop entwining T6SS and QS, thereby coordinating these pathways to enhance the competitive fitness of V. alginolyticus . </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >类型vi分泌系统（T6SS）是可以介导细菌细胞杀死的多素蛋白分泌机，从而改变细菌群落的组成。控制这些杀灭机器的产生和分泌的机制是不完全理解的，尽管仲裁感测（QS）和PPKA激酶在某些生物中调节T6SS活性。在这里，我们研究了对海洋生物的T6s进行了控制，该T6S在编码两个T6SS系统（T6SS1和T6SS2）中进行母线生物的T6S。我们发现有机体主要依赖于T6S2进行间区间竞争。我们进一步进行了磷蛋白酶筛网以鉴定T6SS2连接的PPKA2激酶的底物。鉴定了在T6SS2簇内的PPKA2的底物作为显然未与T6SS相关过程有关的蛋白质。与其他生物类似，PPKA2自动磷酸化对于T6SS2功能至关重要。值得注意的是，在T6SS2簇之外的多肽的磷酸化对T6SS2表达和功能至关重要，因为它增强了也促进T6SS2基因表达的QS的关键调节器的表达。因此，PPKA2控制培养型级联，其介导正调节回路缠绕T6S和Qs，从而协调这些途径以增强 v的竞争性能。 alginolyticus </ i>。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2018年第2期</span><b style="margin: 0 2px;">|</b> <span>共17页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Zhen&option=202" target="_blank" rel="nofollow">Yang Zhen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou Xiaohui&option=202" target="_blank" rel="nofollow">Zhou Xiaohui;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ma Yue&option=202" target="_blank" rel="nofollow">Ma Yue;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhou Mian&option=202" target="_blank" rel="nofollow">Zhou Mian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Waldor Matthew K.&option=202" target="_blank" rel="nofollow">Waldor Matthew K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yuanxing&option=202" target="_blank" rel="nofollow">Zhang Yuanxing;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Qiyao&option=202" target="_blank" rel="nofollow">Wang Qiyao;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237 China;</p> <p>Department of Pathobiology and Veterinary ScienceUniversity of ConnecticutStorrs CT 06269 USA;</p> <p>State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237 China;</p> <p>State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237 China;</p> <p>State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237 China;</p> <p>State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237 China;</p> <p>State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghai 200237 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229217.html">Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen <i xmlns="http://www.wiley.com/namespaces/wiley">Vibrio alginolyticusVibrio alginolyticus</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang 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Serine/threonine kinase PpkA coordinates the interplay between T6SS2 activation and quorum sensing in the marine pathogen Vibrio alginolyticusVibrio alginolyticus

摘要