Biogeographic conservation of the cytosine epigenome in the globally important marine, nitrogen‐fixing cyanobacterium <i xmlns='http://www.wiley.com/namespaces/wiley'>TrichodesmiumTrichodesmium

Walworth Nathan G.; Hutchins David A.; Dolzhenko Egor; Lee Michael D.; Fu Feixue; Smith Andrew D.; Webb Eric A.

摘要

Summary > Cytosine methylation has been shown to regulate essential cellular processes and impact biological adaptation. Despite its evolutionary importance, only a handful of bacterial, genome‐wide cytosine studies have been conducted, with none for marine bacteria. Here, we examine the genome‐wide, C 5 ‐Methyl‐cytosine (m5C) methylome and its correlation to global transcription in the marine nitrogen‐fixing cyanobacterium Trichodesmium . We characterize genome‐wide methylation and highlight conserved motifs across three Trichodesmium isolates and two Trichodesmium metagenomes, thereby identifying highly conserved, novel genomic signatures of potential gene regulation in Trichodesmium . Certain gene bodies with the highest methylation levels correlate with lower expression levels. Several methylated motifs were highly conserved across spatiotemporally separated Trichodesmium isolates, thereby elucidating biogeographically conserved methylation potential. These motifs were also highly conserved in Trichodesmium metagenomic samples from natural populations suggesting them to be potential in situ markers of m5C methylation. Using these data, we highlight predicted roles of cytosine methylation in global cellular metabolism providing evidence for a ‘core’ m5C methylome spanning different ocean regions. These results provide important insights into the m5C methylation landscape and its biogeochemical implications in an important marine N <sub>2</sub> ‐fixer, as well as advancing evolutionary theory examining methylation influences on adaptation. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>概述</ title> >胞嘧啶甲基化已被证明调节必需的细胞过程和影响生物适应。尽管它的进化重要性，但只有少量的细菌，基因组胞嘧啶研究已经进行了，没有用于海洋细菌。在此，我们研究基因组 - 宽的C 5 - 甲基胞嘧啶（M5C）甲基杂物及其与海洋氮固定蓝藻中的全局转录的相关性吡咯粒子锗-i>。我们以基因组甲基化特征在于三个滴度粒子和分离物和两种滴西锗和梅蛋白，从而识别高度保守的，潜在基因调控的高度保守的新基因组特征</ i>。具有最高的甲基化水平的某些基因体与较低的表达水平相关。几种甲基化的基序在瞬间分离的滴度级和分离物中高度保守，从而阐明生物地图保守的甲基化电位。这些基序也高度保守于来自天然群体的次级菌种，表明它们是M5C甲基化的原位潜在的潜在。使用这些数据，我们突出了在全球性细胞代谢中的胞嘧啶甲基化的预测作用，为跨越不同海洋区域的“核心'M5C甲基族的证据提供了证据。这些结果对M5C甲基化景观的重要见解及其在重要的海洋N <亚> 2 </ sub> -Fixer中的生物地球化学意义，以及推进进化理论检查适应性的甲基化影响。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2017年第11期</span><b style="margin: 0 2px;">|</b> <span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Walworth Nathan G.&option=202" target="_blank" rel="nofollow">Walworth Nathan G.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hutchins David A.&option=202" target="_blank" rel="nofollow">Hutchins David A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dolzhenko Egor&option=202" target="_blank" rel="nofollow">Dolzhenko Egor;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lee Michael D.&option=202" target="_blank" rel="nofollow">Lee Michael D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fu Feixue&option=202" target="_blank" rel="nofollow">Fu Feixue;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Smith Andrew D.&option=202" target="_blank" rel="nofollow">Smith Andrew D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Webb Eric A.&option=202" target="_blank" rel="nofollow">Webb Eric A.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> <p>Department of Biological SciencesUniversity of Southern CaliforniaLos Angeles CA 90089 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023229200.html">Biogeographic conservation of the cytosine epigenome in the globally important marine, nitrogen‐fixing cyanobacterium <i xmlns="http://www.wiley.com/namespaces/wiley">TrichodesmiumTrichodesmium</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Walworth Nathan G.&option=202" target="_blank" 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Biogeographic conservation of the cytosine epigenome in the globally important marine, nitrogen‐fixing cyanobacterium TrichodesmiumTrichodesmium

摘要