Viable but non‐culturable and persistence describe the same bacterial stress state

Kim Jun‐Seob; Chowdhury Nityananda; Yamasaki Ryota; Wood Thomas K.

摘要

Summary > Bacteria are often thought of as having two dormant phenotypes: the viable but non‐culturable (VBNC) state and the persister state. Here we investigate the relatedness of the two stress‐induced phenotypes at the single‐cell level and examine cell morphology and quantify cell resuscitation. Using the classic starvation conditions to create VBNC cells, we found that the majority of the remaining Escherichia coli population are spherical, have empty cytosol and fail to resuscitate; however, some of the spherical cells resuscitate immediately (most probably those?with dense cytosol). Critically, all the culturable cells in this starved population became persister cells within 14 days of starvation. We found that the?persister cells initially are rod‐like, have clear but limited membrane damage, can resuscitate immediately and gradually become spherical by aging. After 24 h, only?rod‐shaped persister cells survive, and all the spherical cells lyse. Both cell populations formed under the VBNC‐inducing conditions and the persister conditions are metabolically inactive. Therefore, the bacterial population consists of dead cells and persister cells in the VBNC‐inducing conditions; that is, the non‐lysed particles that do not resuscitate are dead, and the dormant cells that resuscitate are persister cells. Hence, ‘VBNC’ and ‘persister’ describe the same dormant phenotype. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >细菌是经常被认为是有两种休眠表型：可行但非追加（VBNC）状态和普及州。在这里，我们研究了单细胞水平的两个应激诱导表型的相关性，并检查细胞形态并量化细胞复苏。使用经典的饥饿条件来创建VBNC细胞，我们发现大多数剩余的大肠杆菌</ i>种群是球形的，具有空的细胞溶质，并且不能重新播种;然而，一些球形电池立即重悬（最可能是那些？有致密的细胞溶胶）。批判性地，这种饥饿群体中的所有培养细胞在饥饿后14天内成为普及细胞。我们发现，佩戴物细胞最初是棒状，具有透明但有限的膜损伤，可以立即重刺除并通过老化逐渐变为球形。 24小时后，只有？杆状渗漏细胞存活，所有球形细胞粘合。在VBNC诱导条件下形成的两种细胞群和渗透条件是代谢无活性的。因此，细菌种群由VBNC诱导条件中的死细胞和泄漏细胞组成;也就是说，不复苏的非裂解颗粒已经死亡，并且复苏的休眠细胞是泄漏细胞。因此，'VBNC'和“抗置抗体”描述了相同的休眠表型。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2018年第6期</span><b style="margin: 0 2px;">|</b> <span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Jun‐Seob&option=202" target="_blank" rel="nofollow">Kim Jun‐Seob;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chowdhury Nityananda&option=202" target="_blank" rel="nofollow">Chowdhury Nityananda;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamasaki Ryota&option=202" target="_blank" rel="nofollow">Yamasaki Ryota;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wood Thomas K.&option=202" target="_blank" rel="nofollow">Wood Thomas K.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Biochemistry and Molecular BiologyPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Biochemistry and Molecular BiologyPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Biochemistry and Molecular BiologyPennsylvania State University University ParkPA 16802‐4400 USA;</p> <p>Department of Biochemistry and Molecular BiologyPennsylvania State University University ParkPA 16802‐4400 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023228653.html">Viable but non‐culturable and persistence describe the same bacterial stress state</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Jun‐Seob&option=202" target="_blank" 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Alena Popova</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201173102.html">一种细菌群相同步培养的方法及试剂盒</a> <b>[P]</b> . <span> 中国专利： CN101857893B </span> <span> . 2013.05.01</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120106044320.html">一种细菌群相同步培养的方法及试剂盒</a> <b>[P]</b> . <span> 中国专利： CN101857893A </span> <span> . 2010-10-13</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130417703744.html">Stabilized bacterial composition comprising dried cells or dried culture of a viable lactic acid bacterial strain, a pharmaceutical composition comprising the dried cells or the dried culture, a vacuum- dried edible vegetable oil for use in increasing survival of the dried cells or the dried culture and method of increasing survival of the dried cells or the dried culture</a> <b>[P]</b> . <span> 外国专利：  IL216793A </span> <span> . 2014-05-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：稳定的细菌组合物，其包含活的乳酸菌菌株的干细胞或干培养物;药物组合物，其包含干细胞或干培养物;真空干燥的食用植物油，其用于增加干细胞或干培养物的存活率;和增加干细胞或干培养物存活率的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130437679371.html">Water treatment induced charge promotes viable media for proliferation of bacterial cultures</a> <b>[P]</b> . <span> 外国专利：  NZ539368A </span> <span> . 2006-01-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：水处理引起的电荷促进细菌培养基增殖的可行培养基 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130449083094.html">Replicable plasmids, viable cultures of bacterial transformers and methods for the preparation of human w</a> 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