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Screen‐printed Gold Electrodes Functionalized with Grafted p‐Aminobenzoic Acid for the Construction of Electrochemical Immunosensors. Determination of TGF‐β1 Cytokine in Human Plasma

机译：用接枝对氨基苯甲酸官能化的丝网印刷金电极用于构建电化学免疫调菌体。 人血浆中TGF-β1细胞因子的测定

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Abstract >Screen‐printed gold electrodes were modified with grafted p‐aminobezoic acid to provide scaffolds with high loading of surface confined carboxyl groups that can be used to construct electrochemical immunosensors. As a specific application, an immunosensor for the determination of the cytokine transforming growth factor beta 1 (TGF‐β1) is reported. The capture antibody (anti‐TGF) was covalently immobilized on the surface of the prepared disposable scaffold and a sandwich‐type immunoassay was implemented with biotinylated detector antibody (Biotin‐anti‐TGF) and streptavidin labeled with alkaline phosphatase (AP‐Strept). The transduction event was monitored by differential pulse voltammetry after addition of 1‐naphthyl phosphate (1‐NPP) as the enzyme substrate. The different variables affecting the preparation and the analytical behavior of the AP‐Strept‐Biotin‐anti‐TGF‐TGF‐β1‐anti‐TGF‐SPAuE immunosensor were optimized, and the functionalized gold electrodes and the prepared immunosensor were characterized using several techniques. A linear range extending between 0.05 and 3.0?ng/mL TGF‐β1, adequate for the determination of the cytokine in human plasma, and a LOD of 10?pg/mL, better than that claimed for commercial ELISA kits, were achieved. The immunosensor exhibits also a great selectivity against other proteins and an excellent storage ability. The usefulness of the immunosensor was demonstrated by analyzing human plasma spiked with TGF‐β1. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >用接枝的p- aminobezoice酸改性丝网印刷的金电极，以提供高负荷的支架表面限制羧基，可用于构建电化学免疫调菌体。作为特定应用，报道了用于测定细胞因子转化生长因子β1（TGF-β1）的免疫传感器。将捕获抗体（抗TGF）共价固定在制备的一次性支架的表面上，并用生物素化的检测器抗体（生物素-抗TGF）和用碱性磷酸酶（AP链条）标记的链霉抗生物素蛋白来实现夹层型免疫测定。在加入1-萘基磷酸酯（1-NPP）作为酶底物后，通过差分脉冲伏安法监测转导事件。优化影响AP链霉素 - 抗TGF-β1-抗TGF-β1-抗TGF-β1-抗TGF-β1-抗TGF-β1-抗TGF-β1-抗TGF-SKIMAUE免疫传感器的不同变量，并使用几种技术表征官能化的金电极和制备的免疫传感器。在0.05和3.0？Ng / ml TGF-β1之间延伸的线性范围，足以确定人血浆中细胞因子的含量，而且比索取的10×PG / ml更好地达到商业ELISA试剂盒。免疫传感器也表现出对抗其他蛋白质的良好选择性和优异的储存能力。通过分析用TGF-β1分析人血浆来证明免疫传感器的有用性。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第7期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guerrero Sara&option=202" target="_blank" rel="nofollow">Guerrero Sara;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Agüí Lourdes&option=202" target="_blank" rel="nofollow">Agüí Lourdes;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yá?ez‐Sede?o Paloma&option=202" target="_blank" rel="nofollow">Yá?ez‐Sede?o Paloma;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pingarrón José M.&option=202" target="_blank" rel="nofollow">Pingarrón José M.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Analytical Chemistry Faculty of ChemistryUniversity Complutense of Madrid28040- Madrid Spain;</p> <p>Department of Analytical Chemistry Faculty of ChemistryUniversity Complutense of Madrid28040- Madrid Spain;</p> <p>Department of Analytical Chemistry Faculty of ChemistryUniversity Complutense of Madrid28040- Madrid Spain;</p> <p>Department of Analytical Chemistry Faculty of ChemistryUniversity Complutense of Madrid28040- Madrid Spain;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Screen-printed gold electrodes&option=203" rel="nofollow">Screen-printed gold electrodes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cytokines&option=203" rel="nofollow">cytokines;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=transforming growth factor beta 1&option=203" rel="nofollow">transforming growth factor beta 1;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=electrochemical immunosensor&option=203" rel="nofollow">electrochemical immunosensor;</a> </p> <div class="translation"> 机译：丝网印刷的金电极;细胞因子;转化生长因子β1;电化学免疫传感器; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174976.html">Screen‐printed Gold Electrodes Functionalized with Grafted p‐Aminobenzoic Acid for the Construction of Electrochemical Immunosensors. 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