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首页> 外文期刊>Electroanalysis >Electrochemical Detection of SNP in Human Mitochondrial DNA Using Cyclic Primer Extension with Biotinylated Nucletides and Enzymatic Labeling at Disposable Pencil Graphite Electrodes
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Electrochemical Detection of SNP in Human Mitochondrial DNA Using Cyclic Primer Extension with Biotinylated Nucletides and Enzymatic Labeling at Disposable Pencil Graphite Electrodes

机译：用生物素化核苷酸的循环引物延伸和一次性铅笔石墨电极用循环引物延伸的人体线粒体DNA中SNP的电化学检测

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Abstract >A novel method of SNP typing in human mitochondrial DNA utilizing enzymatic labeling and electrochemical detection at disposable pencil graphite electrodes is described. The procedure is based on amplification of DNA stretches by cyclic primer extension (PEx) of SNP‐specific diagnostic primers in a mixture of biotinylated and natural nucleotides. The diagnostic primers are designed to recognize, by its 3'‐terminal nucleotide, the SNP‐site in target template. Under optimized conditions of the PEx reaction, efficient polymerase synthesis of biotin‐labeled strands takes place only in the case of full complementarity between the diagnostic primer and the target SNP site. There is also benefit from introducing many biotin molecules per extended DNA strand, resulting in another level of signal amplification. After adsorption of biotinylated PEx products at the electrode surface, streptavidin‐alkaline phosphatase conjugate was bound to the biotin tags, 1‐naphthol was enzymatically produced and electrochemically detected. Several critical steps and parameters of the assay, including termination of 3’‐OH ends of residual amplification primers, temperature for annealing of diagnostic primers, relative amount of biotinylated deoxynucleoside triphosphate in the PEx mixture and number of PEx cycles were optimized in this study to attain best SNP resolution, and reduction of time needed for the analysis. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</ title> >使用酶促铅笔的人体线粒体DNA中的SNP键入的新方法描述了石墨电极。该方法基于SNP特异性诊断引物的循环引物延伸（PEX）在生物素化的和天然核苷酸的混合物中扩增DNA延伸。诊断引物被设计为通过其3'-末端核苷酸识别靶模板中的SNP部位。在PEX反应的优化条件下，生物素标记的股线的有效聚合酶合成仅在诊断引物与靶SNP位点之间完全互补的情况下进行。还可以从介绍每个延伸的DNA链中引入许多生物素分子，导致另一种信号放大水平。在电极表面的生物素化的PEX产物吸附后，链霉抗生物素蛋白 - 碱性磷酸酶缀合物与生物素标签结合，酶促产生1-萘酚并电化学检测。测定的几个关键步骤和参数，包括终止于3'-OH结束的残留扩增引物，用于退火的诊断引物的温度，在本研究中优化了PEX混合物中的双膦化脱氧核苷三磷酸的相对量，并在本研究中得到了优化的次数获得最佳SNP分辨率，并减少分析所需的时间。</ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第10期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Plucnara Medard&option=202" target="_blank" rel="nofollow">Plucnara Medard;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eksin Ece&option=202" target="_blank" rel="nofollow">Eksin Ece;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Erdem Arzum&option=202" target="_blank" rel="nofollow">Erdem Arzum;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fojta Miroslav&option=202" target="_blank" rel="nofollow">Fojta Miroslav;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of BiophysicsAcademy of Sciences of the Czech Republic v.v.i.Královopolská 135 612?65 Brno Czech Republic;</p> <p>Ege University Faculty of PharmacyAnalytical Chemistry Department35100 Bornova Izmir Turkey;</p> <p>Ege University Faculty of PharmacyAnalytical Chemistry Department35100 Bornova Izmir Turkey;</p> <p>Institute of BiophysicsAcademy of Sciences of the Czech Republic v.v.i.Královopolská 135 612?65 Brno Czech Republic;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=SNP typing&option=203" rel="nofollow">SNP typing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=primer extension&option=203" rel="nofollow">primer extension;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme label&option=203" rel="nofollow">enzyme label;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=electrochemical detection&option=203" rel="nofollow">electrochemical detection;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pencil graphite electrode&option=203" rel="nofollow">pencil graphite electrode;</a> </p> <div class="translation"> 机译：SNP键入;引物延伸;酶标签;电化学检测;铅笔石墨电极; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-cancer_thesis/0201237083040.html">寡核苷酸阵列引物延伸技术在p53基因突变检测中的应用</a> <b>[J]</b> . <span> <a 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SNPs的快速测定法--引物延伸-飞行时间质谱法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许传超&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 许传超</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=蔡贵庆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,蔡贵庆</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=伍新尧&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,伍新尧</a> <span> <a href="/journal-cn-4545/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中山大学学报（自然科学版） </a> </span> <span> . 2003</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chemical-journal-chinese-universities_thesis/0201231580610.html">基于引物延伸反应进行SNP基因分型的电化学方法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=屈瑜&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 屈瑜</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=楚霞&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,楚霞</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐湘民&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐湘民</a> <span> <a href="/journal-cn-10958/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 高等学校化学学报 </a> </span> <span> . 2009</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-taishan-medical-college_thesis/0201273533516.html">两种聚合酶在等位基因特异性引物延伸法检测SNP中的应用比较</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈载鑫&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈载鑫</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=何丹&option=202" target="_blank" rel="nofollow" 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