Molecular diagnosis of Toxoplasma gondii in Greylag goose (Anser anser)

摘要

Thirty samples of Greylag goose (Anser anser) were collected from Al-Dalmage marshes in Wasit province -Iraq. The specimens of tissue was collected from brain and liver and grinded by grinder after treated with liquid nitrogen to prepare 'tissue powder', Genomic DNA purification kit (promega. USA) was used to extraction of sample's DNA according to company instructions and tested by Real time PCR using primers and probe targeting B1 gene of Toxoplasma gondii parasites. There are nine out of 30 (30%)of duck's brain samples was positive. Whereas 14 out of 30 (46.6%) samples of duck's liver was positive.