Effect of Oral Channa striatus in Laparotomy Wound Healing of Malnourished Rats

摘要

Objective: To evaluate the effect of oral Channa striatus in laparotomy wound healing in malnourished rats in terms of tensile strength, microscopic aspect and hydroxyproline measurement. Design: A randomized control trial. Materials and methods: A study was carried out initially to measure appropriate malnourished diet with eight male Wistar Kiyoto rats at the age 90 days, weighing about 300 g to 350 g. It was followed by an experimental study on forty rats that were divided into two groups, control and oral Channa striatus group. They were starved to become malnourished within thirty days. Subsequently, a four centimeters midline laparotomy wound was inflicted on their abdomen. Wound dressing was done every day until they were sacrificed and any changes over the wound were documented. Independent t test was performed to compare the parameters between control and study groups. Results: The desired weight reduction of 10-12% was achieved after given 50% of the normal diet to achieve the malnourished state within day 21 to day 28. The results demonstrated that treating wound with oral Channa striatus produced better healing proven by better tensile strength, higher fibroblast count and hydroxyproline measurement. Conclusion: Oral Channa striatus has properties that render its capable of promoting wound healing activity. Direct correlation with human subject is not possible but it may serve as a platform to explore on laparotomy wound with patient in malnourished state.