Assessment of Genetic Diversity Using RAPD Marker Among Different Varieties of Rice (Oryza sativa)

摘要

Assessment of the extent and distribution of genetic variation in a crop species and its relatives is essential in understanding pattern of diversity and is highly significant for the improvement of many crop species including rice. The genetic divergence among 12 Indian varieties of rice (Ory^a sativa L.) was assessed employing random amplified polymorphic DNA markers. A total of 136 clear score able bands were generated, out of which 87.25% were polymorphic. The total number of amplicon varied from7 to 10 with a mean of 8 amplicon per primer while in case of polymorphic amplicon it varied from 5 to 9 with a mean of 7 polymorphic amplicon per primer. The amplified product size ranged from 0.3 to 1.8 kb. The Jaccard's similarity coefficient valuesranged 0.56 to 0.90 with a mean of 0.70. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters CL1 and CL2. The dendrogram has put all the genotypes in two clusters.CL1 comprises of three varieties with 85 per cent similarity coefficient whereas remaining 9 varieties clustered together in CL2, which is further classified into two sub clusters with 65.50 per cent similarity. Current study highlights genetic diversity among various varieties and attributed to recent human selection pertinent to few genes. The study revealed rich genetic diversity among rice varieties.