Molecular detection of ce:italic>Toxoplasma gondii/ce:italic> in feathered game intended for human consumption in the Czech Republic

摘要

Toxoplasma gondiiis an important ubiquitous protozoan parasite, which can infect almost all warm-blooded vertebrates, including humans. The diagnosis ofT.gondiiinfection is crucial for the prevention, surveillance, and control of its transmission. Here, a triplex real-time PCR assay targeting theB1gene and529repelement was used to determine the presence ofT.gondiiin feathered game (Anas platyrhynchosandPhasianus colchicus) hunted in the Czech Republic. The prevalence ofT.gondiiwas 5.4% in wild ducks (n?=?280) and 3.4% in common pheasants (n?=?350). Additionally, genotyping of 28T.gondii-positive samples revealed the presence of archetypal genotypes II and III as well as non-archetypal genotypes combining both type II and III alleles. Our results suggest that consumption of feathered game could pose a risk ofT.gondiitransmission to humans in the Czech Republic.