Comparative Transcriptome Analyses of Resistant and Susceptible Near-Isogenic Wheat Lines following Inoculation with Blumeria graminis f. sp. tritici

摘要

Powdery mildew is one of the most important diseases of wheat. In this study, the leaf RNA samples of wheat NILs carryingpowdery mildew resistant and susceptible Pm2 alleles (L031 and Chancellor) and its F1 hybrid at two time points (16h and 96hpostinoculation) were used for RNA-seq analysis. We carry comparison between similar materials at different times andbetween different materials at same times. The overlapping DEGs between the dominant phenotypes (L031 and F1 hybrid) andthe recessive phenotype (Chancellor) were 1028 and 2214 DEGs, which were clearly lower than those between thedominant and recessive parents and thus could provide relatively accurate and valuable information. GO and KEGGenrichment analysis of DEGs revealed that other than the expected defense-related genes, differential up- anddownregulation of genes from many other signaling networks were also involved. Comparative transcriptome analysis alsorevealed that early-stage postinoculation is important and suitable time points to study expression profiles and signalingpathways of resistance-related genes following fungal inoculation. qRT-PCR analyses showed highly consistent expressionpatterns of genes with RNA-seq data. The results will aid in the identification of genes and signaling pathways involved inpowdery mildew response in wheat.