The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies

摘要

High throughput genomic assays empower us to study the entire human genome in short time with reasonable cost. Formalinfixed-paraffin-embedded(FFPE)tissue processing remains the most economical approach for longitudinal tissue specimen storage.Therefore, the ability to apply high throughput genomic applications to FFPE specimens can expand clinical assays and discovery.Many studies have measured the accuracy and repeatability of datagenerated from FFPE specimensusing high throughput genomicassays. Together, these studies demonstrate feasibility and provide crucial guidance for future studies using FFPE specimens. Here,we summarize the findings of these studies and discuss the limitations of high throughput data generated from FFPE specimensacross several platforms that include microarray, high throughput sequencing, and Nano String.