ALTERATION OF ASPARTATE TRANSAMINASE (AST), ALANINE TRANSAMINASE (ALT) AND ACID PHOSPHATASE ACTIVITIES IN BUFFALO MILK RELATED TO UDDER HEALTH STATUS

摘要

The present study was designed to correlate the aspartate transaminase (AST), alanine transaminase (ALT) and acid phosphatase activities in buffalo milk related to udder health status. A total of 60 buffaloes were randomly selected irrespective of their age, breed, feeding practice, stage and season of lactation from the herd belonging to Purnadhadi buffalo unit, PGIVAS; Teaching Veterinary Clinical complex, PGIVAS; Veterinary Polyclinic, Akola and also buffaloes of farmers in and around Akola. Afterthorough clinical examination of each udder about 30 mL of milk was collected in sterilized, clean, dry plastic bottles and after careful screening by CMT these were categorized in normal, sub-clinical (1+), sub-clinical (2+), sub-clinical (3+), and clinical group containing 12 animals in each group. The AST, ALT and acid phosphatase activities were measured by spectrophotometric method. The AST, ALT and acid phosphatase activities were found proportional to the severity of the udder infection as detected by the CMT reactions.