In Reply to Torsvik et al. 'Spontaneous Transformation of Stem Cells In Vitro and the Issue of Cross-Contamination'

摘要

We have recently re-tested the STR fingerprinting data in our paper (Int J Biol Sci 2011; 7:892-901). DNA fingerprinting analysis was performed using genomic DNA from hsNSCs (passage 22); T1 cells (passages <50 and >150) and HeLa cells by GeneMapper ID v3.2 (Applied Biosystems, Foster City, CA) for human STR markers. There are two loci of heterozygosity CSF1PO (9,10) and D3S1358 (15,18) in Tl cells (passages <50) compared with the HeLa STR profiles CSF1PO (10) and D3S1358 (18). In addition, four loci variations are seen at CSF1PO (9, 10), D3S1358 (15, 18), TPOX (8, 12) and vWA (18) in Tl cells (passages >150) which are different from those seen in Hela (vWA: 16,18) and (TPOX: 12). Other than that, the similarity between Tl and Hela was >86%, while the similarity between Tl and hsNSC was <14%. Overall, our recent data suggest that Tl cells may have cross-contaminated by HeLa cells.