Photoactivated Selective Release of Droplets from Microwell Arrays

摘要

Droplet microfluidics has enabled a significant reduction in reaction volume and analysis time, which in turn has led to transformative advances in high-capacity screening and assays. By arranging droplets into a static array, it is possible to monitor dynamic events that occur within these microchambers over an extended period of time, facilitating the identification of rare events and cell types. In many instances, it is highly desirable to recover a small number of droplets that contain unique analytes or cells for further analyses; however, few techniques allow for selective recovery of droplets from such an array without using a complex network of physical valves, which also require a large number of control units external to the microfluidic device. In this report, we present photoactivated selective release of droplets from a static microwell array enabled by a photoresponsive polymer layer integrated into the microfluidic device. This photoresponsive layer is placed in between a microwell array that traps a large number of droplets and a PDMS slab with or without a top flow channel that can be used for recovery. By using focused light, the photoresponsive layer can either be punctured for release-up recovery or induced to create a bubble by local heating to selectively push-down release droplets. We show that the photoresponsive layer is optically transparent within the visible spectrum and thus does not interfere with optical observation of droplets. The type of photoacoustic dye and the physical properties of the photoresponsive layer can be engineered to induce either puncture of the photoresponsive layer or pushing of droplets out of the microwell arrays with low thermal impact on the droplets. We believe that the photoresponsive layer will have a broad impact in the field of soft lithography-based microfluidic devices for various applications including photoresponsive valves as well as high-throughput single-cell sequencing.