Preparation and Characterization of Novel Decellularized Dermis Matrix and Hydroxyapatite Composite Membranes for Tissue Engineering Applications

摘要

Decellularized dermis matrix (DDM), obtained by removing cells of skins through a series of disposal, has the advantages of good biocompatibility, biodegradability, low immunogenicity and low toxicity. Hydroxyapatite (HA), as a natural inorganic component of bones and teeth, has the characteristics of osteoconductivity and osteophilicity. In this work, HA was deposited on the surface of DDM to form DDM-HA composite membranes by alternatively soaking the matrix in CaCl_2 and Na_3PO_4 (pH = 10.5) solutions for 0.5 hour. The DDM-HA composite membranes were characterized using SEM, FTIR, XRD, EDS and a tensile test. The results revealed the presence of phosphorite layer on the surface of DDM, and the size and deposition of phosphorite layers were increased with the increasing number of immersion cycles. In addition, the tensile test displayed that the number of soaking cycles had an influence on the mechanical property of the membranes. Rat osteoblastoid cells ROS 17/2.8 were used for the evaluation of the biocompatibility of DDM-HA composite membranes by MTT assay, and the results revealed that the membranes had good biocompatibility and nontoxicity. All of the results suggest that DDM-HA composite membranes might have potential applications in tissue engineering field.