Impact of various physical exposures on Chlorella Sorokiniana microalgae cultivation

摘要

This work presents investigation of impact of the following physical factors: aeration, mixing operation, daylight fluorescent lamp illumination and infrared irradiation on Chlorella Sorokiniana biomass production. Analysis of biomass production under these exposures was carried out using optical density. The calculation of cell number in ml was performed using hemocytometer (Goryaeva chamber). Suspension pH values were controlled during cultivation. It was shown that pH value strongly affects Chlorella Sorokiniana biomass production. Optimum values of medium pH were determined and pH shift towards alkaline region was explained. Morphological studies of Chlorella Sorokiniana population before and after various physical exposures were carried out by microscopy of intravital cells (colored and non-colored). Magnification power was 640 times. It was shown that agglutination and cell precipitation of microalgae Chlorella Sorokiniana took place without aeration. The optimum conditions for microalgae Chlorella Sorokiniana cultivation were chosen aiming at the highest biomass production.