Construction and characterization of a highly reactive chicken-derived single-chain variable fragment (scFv) antibody against Staphylococcus aureus developed with the T7 phage display system

Li Jingquan; Xu Yongping; Wang Xitao; Li Yuan; Wang Lili; Li Xiaoyu

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Construction and characterization of a highly reactive chicken-derived single-chain variable fragment (scFv) antibody against Staphylococcus aureus developed with the T7 phage display system

机译：用T7噬菌体展示系统产生高反应性鸡肉衍生的单链可变片段（SCFV）抗体的高活性鸡肉衍生的单链可变片段（SCFV）抗体

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摘要

The purpose of this study was to construct a single-chain variable fragment (scFv) antibody from chicken egg yolk immunoglobulin (IgY) by means of genetic engineering and subsequent panning for a specific antibody against Staphylococcus aureus.

机译：本研究的目的是通过基因工程和随后的抗金黄色葡萄球菌的特异性抗体构建来自鸡蛋蛋黄免疫球蛋白（IgY）的单链可变片段（SCFV）抗体。

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来源
《International immunopharmacology》 |2016年第null期|共6页
作者
Li Jingquan; Xu Yongping; Wang Xitao; Li Yuan; Wang Lili; Li Xiaoyu;
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作者单位

Dalian Univ Technol Sch Life Sci &

Biotechnol 2 Linggong Rd Dalian 116024 Peoples R China;

Dalian Univ Technol Sch Life Sci &

Biotechnol 2 Linggong Rd Dalian 116024 Peoples R China;

Dalian Univ Technol Sch Life Sci &

Biotechnol 2 Linggong Rd Dalian 116024 Peoples R China;

Dalian Univ Technol Sch Life Sci &

Biotechnol 2 Linggong Rd Dalian 116024 Peoples R China;

Dalian Univ Technol Sch Life Sci &

Biotechnol 2 Linggong Rd Dalian 116024 Peoples R China;

Dalian Univ Technol Sch Life Sci &

Biotechnol 2 Linggong Rd Dalian 116024 Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类药理学;
关键词
Chicken IgY; Phage display; Staphylococcus aureus; Single-chain variable fragment (scFv);

机译：鸡IGY;噬菌体展示;金黄色葡萄球菌;单链可变片段（SCFV）;
入库时间 2022-08-20 02:01:14

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专利

1. Construction and characterization of a highly reactive chicken-derived single-chain variable fragment (scFv) antibody against Staphylococcus aureus developed with the T7 phage display system [J] . Li Jingquan, Xu Yongping, Wang Xitao, International immunopharmacology . 2016,第Null期

机译：用T7噬菌体展示系统开发的高反应性鸡源性抗金黄色葡萄球菌单链可变片段（scFv）抗体的构建和表征
2. Construction and characterization of a highly reactive chicken-derived single-chain variable fragment (scFv) antibody against Staphylococcus aureus developed with the T7 phage display system [J] . Li Jingquan, Xu Yongping, Wang Xitao, International immunopharmacology . 2016,第Null期

机译：用T7噬菌体展示系统产生高反应性鸡肉衍生的单链可变片段（SCFV）抗体的高活性鸡肉衍生的单链可变片段（SCFV）抗体
3. Generation of a Highly Reactive Chicken-Derived Single-Chain Variable Fragment against Fusarium verticillioides by Phage Display [J] . Greta N#x000F6, lke, He-Ping Li, International Journal of Molecular Sciences . 2012,第6期

机译：通过噬菌体展示的高活性鸡源性单链可变链片段抗小镰刀菌的产生。
4. Increasing the solubility of single-chain variable fragment (scFv) antibody produced in recombinant Escherichia coli by fed-batch culture with DO-stat. [C] . Nghia N.H., Thu N.P.A., Kishimoto M. 化学工学会研究講演発表会講演要旨集 . 2019

机译：通过用DO-STAT通过FED分批培养增加重组大肠杆菌中的单链可变片段（SCFV）抗体的溶解度。
5. Construction and characterization of single chain variable fragment antibodies against C-type lectin receptor DEC 205 and complement receptor 1 for Streptococcus gordonii targeting to dendritic cells. [D] . Knight, Jennifer B. 2005

机译：针对戈登链球菌靶向树突细胞的C型凝集素受体DEC 205和补体受体1的单链可变片段抗体的构建和表征。
6. Generation of a Highly Reactive Chicken-Derived Single-Chain Variable Fragment against Fusarium verticillioides by Phage Display [O] . Zu-Quan Hu, Jin-Long Liu, He-Ping Li, 2012

机译：通过噬菌体展示的高活性鸡源性单链可变片段抗小镰刀菌
7. Generation of a Highly Reactive Chicken-Derived Single-Chain Variable Fragment against <em>Fusarium verticillioides</em> by Phage Display [O] . Zu-Quan Hu, Jin-Long Liu, He-Ping Li, 2012

机译：产生针对＆lt; em＆gt; Fusarium verticillioides的高反应性鸡衍生的单链可变片段＆lt; / em＆gt;通过phage Display

Construction and characterization of a highly reactive chicken-derived single-chain variable fragment (scFv) antibody against Staphylococcus aureus developed with the T7 phage display system

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