Biotechnological approach for alpha-pinene, beta-pinene, and Delta 3-carene degradation in pine wood for reduced terpene emissions from Oriented Cheek for Strand Boards

摘要

This study investigated the reduction of terpene emissions from Oriented Strand Boards (OSB) by pre-treatment of pine wood strands with a mixed culture of Pseudomonas bacteria and the ascomycete Penicillium nigricans. While Pseudomonas alone efficiently degraded alpha-pinene and beta-pinene, the combined action of both microorganisms was required for the simultaneous degradation of all three major terpenes in pine wood including Delta 3-carene. Delta 3-carene emissions from laboratory OSB were 60% and 35% lower than that of controls after 4 and 2 days of microbial pre-treatment, respectively. At the same time, alpha-pinene emissions were reduced by 85% and 45%, while beta-pinene emissions were completely absent. Delta 3-carene degradation was no longer observed at a short-term treatment of only 1 day. However, laccase in the presence of a redox mediator may overcome this limitation by oxidation of Delta 3-carene to non-volatile carenones. The results revealed the potential of biotechnology for reducing potentially harmful terpene emissions from wood products.