In Vitro Regeneration Studies in Brassica napus with Response to Callus Induction Frequency and Regeneration Frequency

摘要

The present study entitled "In vitro Regeneration Studies in Brassica napus, variety GSL-1 and DGS-1 was carried out in the Biotechnology Laboratory, Division of Genetics and Plant Breeding SKUAST-J. Brassica napus, variety GSL 1 and DGS-1 were cultured on MS Media supplemented with different concentrations of growth regulators. The sterilization treatment with HgCl2 (0.1%) for 3 minutes resulted in highest per cent of aseptic seeds and survival of seedlings. Highest callus induction was observed inGSL-1. MS medium augmented with 2,4-D at 2-2.5 mg/1. Different concentrations of growth regulators in media showed a significant difference in the callus induction frequency (CIF) and regeneration frequency (RF) for both the genotypes tested. MS medium supplemented with BAP (5.0 mg/1) and 2, 4-D (0.5 mg/1) showed highest shoot regeneration frequency in GSL-1.