miR-155 for diagnosing thromboangiitis obliterans and its effect on platelet function: a clinical study

摘要

Objective: To investigate the effect of miR-155 on the diagnosis of thromboangiitis obliterans and on platelet function. Method: A prospective study was conducted among 88 patients with thromboangiitis obliterans (TAO group) and 90 healthy subjects (control group). The relative expression levels of serum miR-155, P-selectin (CD62p) and platelet activated complex-1 (PAC-1) were measured. Results: The relative expression of miR-155 in the TAO group was significantly higher than that of the control group. Receiver operator characteristics analysis showed that the optimal diagnostic threshold of miR-155 was 22.82, with Yoden index of 79.6%, sensitivity of 81.8%, and specificity of 97.8%. As the stage of thromboangiitis obliterans got advanced, the serum level of miR-155 in patients became higher. The expression levels of miR-155 in patients at three stages were all higher than those in the healthy control subjects (all P<0.01). As the stage of thromboangiitis obliterans got advanced, higher expression levels of CD62p and PAC-1 were detected in serum (both P<0.001). Pearson correlation coefficients showed that serum miR155 was positively correlated with CD62p and PAC-1(r=0.637, r=0.620, both P<0.001). Conclusion: The miR-155 is highly expressed in patients with thromboangiitis obliterans, so it is valuable for the diagnosis. Moreover, miR-155 can lead to the occurrence or progression of thromboangiitis obliterans, and may be related to the expressions of CD62p and PAC-1.