Development of a Novel Monoclonal Antibody-Based Indirect Competitive ELISA with Immunoaffinity Cleanup for the Detection of Triclosan in Chickens

摘要

Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high sensitivity and specificity in phosphate-buffered saline buffer, with a half maximal inhibition concentration value of 1.77 ng mL(-1) and a limit of detection of 0.09 ng mL(-1). The average recovery in spiked chicken muscle samples was 84.4-114.8%, with a relative standard deviation below 8.1%. To validate the developed icELISA method, samples spiked with different levels of TCS were analyzed by icELISA and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The consistency of results detected by icELISA and UPLC-MS/MS demonstrated that immunoaffinity cleanup followed by icELISA could also be used for other food matrices.